This review provides an overview of the biomedical applications of natural polysaccharide hydrogels and their derivatives, focusing on their fabrication, physicochemical properties, and biomedical applications. Polysaccharide hydrogels, such as chitosan, hyaluronic acid (HA), alginate, and cellulose, are highlighted for their stability, biocompatibility, and biodegradability. These materials are explored in various processing techniques, including electrospinning, microneedles, microspheres, and nanogels. The review discusses the hemostatic, antimicrobial, anti-inflammatory, and proangiogenic properties of polysaccharide hydrogels in wound healing. Challenges in the development of polysaccharide hydrogels are addressed, along with potential future prospects in this evolving field. The article also delves into the classification and crosslinking mechanisms of polysaccharides, emphasizing the importance of understanding these aspects for optimizing their biomedical applications. Advanced processing technologies, such as 3D printing, electrospinning, and microneedles, are discussed for their role in enhancing the functionality and therapeutic efficacy of polysaccharide-based hydrogels.This review provides an overview of the biomedical applications of natural polysaccharide hydrogels and their derivatives, focusing on their fabrication, physicochemical properties, and biomedical applications. Polysaccharide hydrogels, such as chitosan, hyaluronic acid (HA), alginate, and cellulose, are highlighted for their stability, biocompatibility, and biodegradability. These materials are explored in various processing techniques, including electrospinning, microneedles, microspheres, and nanogels. The review discusses the hemostatic, antimicrobial, anti-inflammatory, and proangiogenic properties of polysaccharide hydrogels in wound healing. Challenges in the development of polysaccharide hydrogels are addressed, along with potential future prospects in this evolving field. The article also delves into the classification and crosslinking mechanisms of polysaccharides, emphasizing the importance of understanding these aspects for optimizing their biomedical applications. Advanced processing technologies, such as 3D printing, electrospinning, and microneedles, are discussed for their role in enhancing the functionality and therapeutic efficacy of polysaccharide-based hydrogels.