Polysaccharide hydrogels are promising materials for skin wound healing due to their biocompatibility, biodegradability, and ability to support tissue regeneration. This review discusses the biomedical applications of natural polysaccharide hydrogels and their derivatives, focusing on their roles in wound healing, including hemostasis, antimicrobial activity, anti-inflammatory effects, and promotion of angiogenesis. Common polysaccharides used in wound healing include chitosan (CS), hyaluronic acid (HA), and alginate, each with unique properties that make them suitable for various wound care applications. CS exhibits antimicrobial and hemostatic properties, HA enhances tissue hydration and cell migration, and alginate provides a moist environment for wound healing. These hydrogels can be fabricated using various techniques, such as electrospinning, microneedles, and microspheres, to create structures that support cell adhesion, proliferation, and tissue repair. The crosslinking mechanisms of polysaccharides, including physical and chemical crosslinking, are crucial for controlling the mechanical strength, swelling behavior, and degradation rate of hydrogels. Double-network hydrogels, which combine natural and synthetic polysaccharides, offer enhanced mechanical properties and are suitable for advanced wound healing applications. The review also highlights the importance of 3D printing and electrospinning in fabricating customized hydrogel scaffolds for tissue engineering and regenerative medicine. Microneedles, which are minimally invasive, offer a promising approach for drug delivery and localized therapy in wound healing. Overall, polysaccharide-based hydrogels show great potential in improving wound healing outcomes due to their unique properties and versatile applications in biomedical fields.Polysaccharide hydrogels are promising materials for skin wound healing due to their biocompatibility, biodegradability, and ability to support tissue regeneration. This review discusses the biomedical applications of natural polysaccharide hydrogels and their derivatives, focusing on their roles in wound healing, including hemostasis, antimicrobial activity, anti-inflammatory effects, and promotion of angiogenesis. Common polysaccharides used in wound healing include chitosan (CS), hyaluronic acid (HA), and alginate, each with unique properties that make them suitable for various wound care applications. CS exhibits antimicrobial and hemostatic properties, HA enhances tissue hydration and cell migration, and alginate provides a moist environment for wound healing. These hydrogels can be fabricated using various techniques, such as electrospinning, microneedles, and microspheres, to create structures that support cell adhesion, proliferation, and tissue repair. The crosslinking mechanisms of polysaccharides, including physical and chemical crosslinking, are crucial for controlling the mechanical strength, swelling behavior, and degradation rate of hydrogels. Double-network hydrogels, which combine natural and synthetic polysaccharides, offer enhanced mechanical properties and are suitable for advanced wound healing applications. The review also highlights the importance of 3D printing and electrospinning in fabricating customized hydrogel scaffolds for tissue engineering and regenerative medicine. Microneedles, which are minimally invasive, offer a promising approach for drug delivery and localized therapy in wound healing. Overall, polysaccharide-based hydrogels show great potential in improving wound healing outcomes due to their unique properties and versatile applications in biomedical fields.