Positive associations among soil organisms are widespread and crucial for maintaining global soil biodiversity and ecological networks. A global survey of soil ecosystems across 20 regions revealed that positive associations between soil taxa, including bacteria, fungi, protists, and invertebrates, dominate soil ecological networks. These associations support larger biodiversity and enhance network stability against species loss. The study highlights that positive interactions, such as facilitative motifs, are prevalent in soil networks, suggesting that cooperation among soil organisms is essential for maintaining soil biodiversity and ecosystem functions. The findings indicate that soil networks with higher positive associations are more resilient to environmental changes. The research also shows that temperature seasonality is strongly correlated with the prevalence of positive associations in soil networks, emphasizing the role of climate in shaping soil ecological structures. The study underscores the importance of positive associations in maintaining soil biodiversity and ecosystem stability, providing new insights into the role of soil organisms in global ecological networks. The results suggest that cooperative interactions among soil taxa are key to sustaining complex soil ecosystems and supporting the multifunctionality of soil systems. The study addresses key knowledge gaps in understanding the role of ecological networks in maintaining soil biodiversity and highlights the need for further research into the mechanisms driving these associations. Overall, the findings emphasize the significance of positive associations in shaping global soil ecosystems and their resilience to environmental changes.Positive associations among soil organisms are widespread and crucial for maintaining global soil biodiversity and ecological networks. A global survey of soil ecosystems across 20 regions revealed that positive associations between soil taxa, including bacteria, fungi, protists, and invertebrates, dominate soil ecological networks. These associations support larger biodiversity and enhance network stability against species loss. The study highlights that positive interactions, such as facilitative motifs, are prevalent in soil networks, suggesting that cooperation among soil organisms is essential for maintaining soil biodiversity and ecosystem functions. The findings indicate that soil networks with higher positive associations are more resilient to environmental changes. The research also shows that temperature seasonality is strongly correlated with the prevalence of positive associations in soil networks, emphasizing the role of climate in shaping soil ecological structures. The study underscores the importance of positive associations in maintaining soil biodiversity and ecosystem stability, providing new insights into the role of soil organisms in global ecological networks. The results suggest that cooperative interactions among soil taxa are key to sustaining complex soil ecosystems and supporting the multifunctionality of soil systems. The study addresses key knowledge gaps in understanding the role of ecological networks in maintaining soil biodiversity and highlights the need for further research into the mechanisms driving these associations. Overall, the findings emphasize the significance of positive associations in shaping global soil ecosystems and their resilience to environmental changes.