The study investigates the functional architecture of the precuneus in both humans and macaque monkeys using resting-state functional MRI (fMRI). Three distinct patterns of functional connectivity were identified within the precuneus: (i) the anterior precuneus, which is functionally connected with sensorimotor regions; (ii) the central precuneus, which is connected to cognitive/associative regions; and (iii) the posterior precuneus, which displays connections with visual cortical regions. These patterns were consistent with anatomical studies in monkeys and suggest that the precuneus is functionally heterogeneous. The findings support the use of resting-state functional connectivity (RSFC) as a tool for cross-species comparisons of functional neuroanatomy and highlight the importance of considering the functional subdivisions within the precuneus in neuroimaging studies.The study investigates the functional architecture of the precuneus in both humans and macaque monkeys using resting-state functional MRI (fMRI). Three distinct patterns of functional connectivity were identified within the precuneus: (i) the anterior precuneus, which is functionally connected with sensorimotor regions; (ii) the central precuneus, which is connected to cognitive/associative regions; and (iii) the posterior precuneus, which displays connections with visual cortical regions. These patterns were consistent with anatomical studies in monkeys and suggest that the precuneus is functionally heterogeneous. The findings support the use of resting-state functional connectivity (RSFC) as a tool for cross-species comparisons of functional neuroanatomy and highlight the importance of considering the functional subdivisions within the precuneus in neuroimaging studies.