This article describes a glycan microarray constructed using standard robotic printing technology to couple amine-functionalized glycans to an amino-reactive glass slide. The array includes 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array is highly effective for profiling the specificity of a wide range of glycan binding proteins (GBPs), including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, plant and microbial lectins, and intact viruses. The array's utility is demonstrated through the analysis of various GBPs, such as DC-SIGN, CD22, and galectin-4, as well as anticarbohydrate antibodies and bacterial and viral GBPs. The array's ability to detect a diverse range of antibody specificities in crude human serum highlights its potential for diagnostic applications in microbial infections, cancer, autoimmune diseases, and xenotransplantation. The study also discusses the importance of multivalency in enhancing the detection of GBPs and the potential therapeutic applications of the array.This article describes a glycan microarray constructed using standard robotic printing technology to couple amine-functionalized glycans to an amino-reactive glass slide. The array includes 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array is highly effective for profiling the specificity of a wide range of glycan binding proteins (GBPs), including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, plant and microbial lectins, and intact viruses. The array's utility is demonstrated through the analysis of various GBPs, such as DC-SIGN, CD22, and galectin-4, as well as anticarbohydrate antibodies and bacterial and viral GBPs. The array's ability to detect a diverse range of antibody specificities in crude human serum highlights its potential for diagnostic applications in microbial infections, cancer, autoimmune diseases, and xenotransplantation. The study also discusses the importance of multivalency in enhancing the detection of GBPs and the potential therapeutic applications of the array.