This article presents a novel method for bioprinting three-dimensional tissue analogues using decellularized extracellular matrix (dECM) bioink. The method enables the creation of cell-laden constructs with an optimized microenvironment that supports the growth and function of three-dimensional structured tissue. The study demonstrates the versatility of the bioprinting process using tissue-specific dECM bioinks, including adipose, cartilage, and heart tissues, which provide crucial cues for cell engraftment, survival, and long-term function. The bioprinting method allows for the fabrication of highly open porous 3D structures, which are essential for nutrient and oxygen supply. The study also shows that the dECM bioink can support the intrinsic cellular morphologies and functions of living tissues, as well as the formation of tissue-specific gene expression and structural maturation. The dECM bioink is prepared through a combination of physical, chemical, and enzymatic processes, and is then solubilized and adjusted to physiological pH for printing. The dECM bioink is capable of retaining its 3D structure after gelation at physiological temperature. The study also demonstrates the ability of the bioprinting method to produce cell-laden constructs with high cell viability and functionality, as well as the potential for tissue-specific differentiation and maturation. The study highlights the importance of using tissue-specific ECMs to recreate the natural microenvironment of cells and to support their function and survival. The results show that the dECM bioink can support the differentiation of stem cells into specific lineages, such as adipogenic, chondrogenic, and cardiogenic, and can also support the structural maturation of myoblasts. The study also demonstrates the potential of the bioprinting method for applications in tissue engineering, in vitro drug screening, and tissue/cancer models. The method is capable of producing constructs with various geometries and can be used to fabricate composite tissues. The study also shows that the bioprinting method is capable of producing constructs with high cell viability and functionality, and that the dECM bioink can support the survival and proliferation of cells. The study concludes that the use of dECM bioink in bioprinting is an attractive option for both in vitro and in vivo tissue reconstruction, and that the method has the potential for a wide range of applications in tissue engineering and regenerative medicine.This article presents a novel method for bioprinting three-dimensional tissue analogues using decellularized extracellular matrix (dECM) bioink. The method enables the creation of cell-laden constructs with an optimized microenvironment that supports the growth and function of three-dimensional structured tissue. The study demonstrates the versatility of the bioprinting process using tissue-specific dECM bioinks, including adipose, cartilage, and heart tissues, which provide crucial cues for cell engraftment, survival, and long-term function. The bioprinting method allows for the fabrication of highly open porous 3D structures, which are essential for nutrient and oxygen supply. The study also shows that the dECM bioink can support the intrinsic cellular morphologies and functions of living tissues, as well as the formation of tissue-specific gene expression and structural maturation. The dECM bioink is prepared through a combination of physical, chemical, and enzymatic processes, and is then solubilized and adjusted to physiological pH for printing. The dECM bioink is capable of retaining its 3D structure after gelation at physiological temperature. The study also demonstrates the ability of the bioprinting method to produce cell-laden constructs with high cell viability and functionality, as well as the potential for tissue-specific differentiation and maturation. The study highlights the importance of using tissue-specific ECMs to recreate the natural microenvironment of cells and to support their function and survival. The results show that the dECM bioink can support the differentiation of stem cells into specific lineages, such as adipogenic, chondrogenic, and cardiogenic, and can also support the structural maturation of myoblasts. The study also demonstrates the potential of the bioprinting method for applications in tissue engineering, in vitro drug screening, and tissue/cancer models. The method is capable of producing constructs with various geometries and can be used to fabricate composite tissues. The study also shows that the bioprinting method is capable of producing constructs with high cell viability and functionality, and that the dECM bioink can support the survival and proliferation of cells. The study concludes that the use of dECM bioink in bioprinting is an attractive option for both in vitro and in vivo tissue reconstruction, and that the method has the potential for a wide range of applications in tissue engineering and regenerative medicine.