The article presents a novel method for bioprinting three-dimensional (3D) tissue analogues using decellularized extracellular matrix (dECM) bioink. The authors developed a bioprinting process that can encapsulate living cells within dECM to create structures resembling natural tissues. The versatility and flexibility of this method are demonstrated using dECMs from adipose, cartilage, and heart tissues, which provide crucial cues for cell engraftment, survival, and long-term function. The bioprinting process involves decellularizing the tissues, solubilizing the dECM in acidic conditions, and adjusting the pH to physiological levels before encapsulating living cells. The resulting dECM pre-gels exhibit shear-thinning behavior and gel at 37°C, forming stable 3D structures. The printed constructs show high cell viability and functionality, with cells maintaining their morphology and function. The study also reports on the differentiation of stem cells into specific lineages (chondrogenic, cardiogenic, and adipogenic) within the dECM constructs, highlighting the potential of this method for tissue engineering and regenerative medicine.The article presents a novel method for bioprinting three-dimensional (3D) tissue analogues using decellularized extracellular matrix (dECM) bioink. The authors developed a bioprinting process that can encapsulate living cells within dECM to create structures resembling natural tissues. The versatility and flexibility of this method are demonstrated using dECMs from adipose, cartilage, and heart tissues, which provide crucial cues for cell engraftment, survival, and long-term function. The bioprinting process involves decellularizing the tissues, solubilizing the dECM in acidic conditions, and adjusting the pH to physiological levels before encapsulating living cells. The resulting dECM pre-gels exhibit shear-thinning behavior and gel at 37°C, forming stable 3D structures. The printed constructs show high cell viability and functionality, with cells maintaining their morphology and function. The study also reports on the differentiation of stem cells into specific lineages (chondrogenic, cardiogenic, and adipogenic) within the dECM constructs, highlighting the potential of this method for tissue engineering and regenerative medicine.