The article reviews the recent advancements in using pristine metal-organic frameworks (MOFs) as modification layers for separators in lithium-sulfur (Li-S) batteries. MOFs, known for their porous structure, high adsorption capacity, and structural flexibility, have emerged as ideal materials to address the severe polysulfides shuttle effect and sluggish sulfur redox kinetics in Li-S batteries. The review covers various design strategies for MOFs, including pore environment modification, metal site construction, conductive MOF design, morphology control, and MOF-carbon or MOF-polymer composites. Each strategy is explained in detail, highlighting the mechanisms behind enhanced electrochemical performance. The article also discusses the challenges and future research directions, emphasizing the need for improved Li⁺ conductivity and the development of multifunctional MOFs to enhance polysulfide interception and rapid Li⁺ conduction. The review concludes with a comprehensive outlook on the potential of MOFs in advancing Li-S battery technology.The article reviews the recent advancements in using pristine metal-organic frameworks (MOFs) as modification layers for separators in lithium-sulfur (Li-S) batteries. MOFs, known for their porous structure, high adsorption capacity, and structural flexibility, have emerged as ideal materials to address the severe polysulfides shuttle effect and sluggish sulfur redox kinetics in Li-S batteries. The review covers various design strategies for MOFs, including pore environment modification, metal site construction, conductive MOF design, morphology control, and MOF-carbon or MOF-polymer composites. Each strategy is explained in detail, highlighting the mechanisms behind enhanced electrochemical performance. The article also discusses the challenges and future research directions, emphasizing the need for improved Li⁺ conductivity and the development of multifunctional MOFs to enhance polysulfide interception and rapid Li⁺ conduction. The review concludes with a comprehensive outlook on the potential of MOFs in advancing Li-S battery technology.