This study investigates the prognostic relevance and validation of ARPC1A in the progression of low-grade glioma (LGG). The authors screened several mitochondrial-related genes, including SLBP, COMM7, LSM4, TOMM34, RPP40, FKBP1A, ARPC1A, and TBCA, using bioinformatics analysis and RT-PCR experiments. They constructed a nomogram to evaluate the clinical relevance of risk factors such as age, WHO grade, IDH mutation status, Ch.1p19q co-deletion status, and high and low expression of ARPC1A in predicting 1-, 3-, and 5-year overall survival. Gene set enrichment analysis was performed to elucidate the cancer-promoting pathways involved in LGG through KEGG and GO analysis. Transfection assays, CCK-8 assays, and flow cytometry were used to determine the proliferation rate and apoptosis rate of HS683 and SW1783 cell lines. Western blotting was employed to examine the involvement of ARPC1A in the MAPK signaling pathway. The results indicate that ARPC1A has a significant prognostic value in LGG, may be a significant independent risk factor, and promotes LGG proliferation by controlling the MAP kinase signaling pathway. Future studies are needed to explore its clinical implications.This study investigates the prognostic relevance and validation of ARPC1A in the progression of low-grade glioma (LGG). The authors screened several mitochondrial-related genes, including SLBP, COMM7, LSM4, TOMM34, RPP40, FKBP1A, ARPC1A, and TBCA, using bioinformatics analysis and RT-PCR experiments. They constructed a nomogram to evaluate the clinical relevance of risk factors such as age, WHO grade, IDH mutation status, Ch.1p19q co-deletion status, and high and low expression of ARPC1A in predicting 1-, 3-, and 5-year overall survival. Gene set enrichment analysis was performed to elucidate the cancer-promoting pathways involved in LGG through KEGG and GO analysis. Transfection assays, CCK-8 assays, and flow cytometry were used to determine the proliferation rate and apoptosis rate of HS683 and SW1783 cell lines. Western blotting was employed to examine the involvement of ARPC1A in the MAPK signaling pathway. The results indicate that ARPC1A has a significant prognostic value in LGG, may be a significant independent risk factor, and promotes LGG proliferation by controlling the MAP kinase signaling pathway. Future studies are needed to explore its clinical implications.