2017; 7(3): 789-804. doi: 10.7150/thno.18133 | Pin Li, Melisa Kaslan, Sze Han Lee, Justin Yao, and Zhiqiang Gao
The article "Progress in Exosome Isolation Techniques" by Pin Li, Melisa Kaslan, Sze Han Lee, Justin Yao, and Zhiqiang Gao reviews the advancements in exosome isolation methods. Exosomes, small membrane vesicles secreted by various cells, play crucial roles in cell communication and are associated with the pathogenesis of human malignancies, making them valuable biomarkers for disease diagnosis, prognosis, and therapy. The authors highlight the need for simple, efficient, and affordable isolation techniques and discuss five main groups of exosome isolation methods: differential ultracentrifugation, size-based techniques, immunoaffinity capture, exosome precipitation, and microfluidics-based techniques. Each method is evaluated based on its mechanism, performance, challenges, and future prospects. The article emphasizes the importance of these techniques in advancing the understanding and clinical applications of exosomes, while also addressing the limitations and ongoing challenges in the field.The article "Progress in Exosome Isolation Techniques" by Pin Li, Melisa Kaslan, Sze Han Lee, Justin Yao, and Zhiqiang Gao reviews the advancements in exosome isolation methods. Exosomes, small membrane vesicles secreted by various cells, play crucial roles in cell communication and are associated with the pathogenesis of human malignancies, making them valuable biomarkers for disease diagnosis, prognosis, and therapy. The authors highlight the need for simple, efficient, and affordable isolation techniques and discuss five main groups of exosome isolation methods: differential ultracentrifugation, size-based techniques, immunoaffinity capture, exosome precipitation, and microfluidics-based techniques. Each method is evaluated based on its mechanism, performance, challenges, and future prospects. The article emphasizes the importance of these techniques in advancing the understanding and clinical applications of exosomes, while also addressing the limitations and ongoing challenges in the field.