The Protein Data Bank (PDB), initiated in 1971 by researchers including Edgar Meyer, Gerson Coheon, and Helen M Berman, is a major international resource for structural biology. It contains over 83,768 entries, including proteins, nucleic acids, and other biological molecules. The PDB provides three-dimensional structural data of biological macromolecules determined by methods such as X-ray crystallography, NMR spectroscopy, and electron microscopy. The database is freely accessible and maintained by the Research Collaborators for Structural Bioinformatics (RCBS) at Rutgers and UCSD, funded by various agencies including the NSF, NIGMS, and NLM. The PDB website supports multiple operating systems and requires a modern web browser with JavaScript and cookies enabled. Users can search for structures based on various criteria, including authors, macromolecules, sequence, and ligand. The database offers downloadable, viewable, and 3D-representable files in formats such as PDB, mmCIF, and PDBML. The PDB also hosts educational resources like PDB-101 and provides tools for structure comparison and data retrieval. The PDB collaborates with other organizations, including PDBe, PDBj, and BMRB, to form the Worldwide Protein Data Bank (wwPDB), which aims to maintain a single, publicly accessible archive of macromolecular structural data. The wwPDB's mission is to ensure global access to structural biological data. The PDB also provides residue-level mapping through SIFTS, an authoritative source for structural annotations. The PDB is a critical resource for researchers in structural biology, offering extensive data and tools for analysis and visualization.The Protein Data Bank (PDB), initiated in 1971 by researchers including Edgar Meyer, Gerson Coheon, and Helen M Berman, is a major international resource for structural biology. It contains over 83,768 entries, including proteins, nucleic acids, and other biological molecules. The PDB provides three-dimensional structural data of biological macromolecules determined by methods such as X-ray crystallography, NMR spectroscopy, and electron microscopy. The database is freely accessible and maintained by the Research Collaborators for Structural Bioinformatics (RCBS) at Rutgers and UCSD, funded by various agencies including the NSF, NIGMS, and NLM. The PDB website supports multiple operating systems and requires a modern web browser with JavaScript and cookies enabled. Users can search for structures based on various criteria, including authors, macromolecules, sequence, and ligand. The database offers downloadable, viewable, and 3D-representable files in formats such as PDB, mmCIF, and PDBML. The PDB also hosts educational resources like PDB-101 and provides tools for structure comparison and data retrieval. The PDB collaborates with other organizations, including PDBe, PDBj, and BMRB, to form the Worldwide Protein Data Bank (wwPDB), which aims to maintain a single, publicly accessible archive of macromolecular structural data. The wwPDB's mission is to ensure global access to structural biological data. The PDB also provides residue-level mapping through SIFTS, an authoritative source for structural annotations. The PDB is a critical resource for researchers in structural biology, offering extensive data and tools for analysis and visualization.