The article describes a method for the partial purification of large quantities of functional, poly(A)-rich mRNA using oligo(dT)-cellulose chromatography. The technique involves annealing poly(A)-rich mRNA to oligo(dT)-cellulose columns and eluting it with buffers of low ionic strength. The method was successfully applied to purify biologically active rabbit globin mRNA, which was then assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor cells. The study highlights the advantages of using oligo(dT)-cellulose for separating globin mRNA from ribosomal RNA in crude polysomal extracts. The purified mRNA was found to be rich in poly(A) and could be translated in the ascites cell-free extract. The authors also discuss the potential of this approach for isolating specific mRNAs and its utility in various biological and biochemical applications.The article describes a method for the partial purification of large quantities of functional, poly(A)-rich mRNA using oligo(dT)-cellulose chromatography. The technique involves annealing poly(A)-rich mRNA to oligo(dT)-cellulose columns and eluting it with buffers of low ionic strength. The method was successfully applied to purify biologically active rabbit globin mRNA, which was then assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor cells. The study highlights the advantages of using oligo(dT)-cellulose for separating globin mRNA from ribosomal RNA in crude polysomal extracts. The purified mRNA was found to be rich in poly(A) and could be translated in the ascites cell-free extract. The authors also discuss the potential of this approach for isolating specific mRNAs and its utility in various biological and biochemical applications.