This study introduces a novel and efficient method for the capture and quantitative assessment of migrasomes, a type of extracellular microvesicle, using wheat germ agglutinin (WGA)-coated magnetic beads and flow cytometry (WBFC). Migrasomes are released by migrating cells and contain various RNAs and proteins, making them valuable for disease diagnosis through liquid biopsy. The researchers validated the WBFC method by analyzing migrasomes in cell culture medium, serum, and urine samples from kidney disease (KD) patients with podocyte injury and healthy volunteers. The results showed a significant increase in urinary podocyte-derived migrasome concentrations in KD patients compared to healthy individuals. Notably, these migrasomes expressed high levels of phospholipase A2 receptor (PLA2R) proteins, which could serve as a natural antigen for quantifying autoantibodies against PLA2R in the serum of patients with membranous nephropathy (MN). The study highlights the potential of urinary migrasomes as a promising biomarker for early diagnosis of KD with podocyte injury.This study introduces a novel and efficient method for the capture and quantitative assessment of migrasomes, a type of extracellular microvesicle, using wheat germ agglutinin (WGA)-coated magnetic beads and flow cytometry (WBFC). Migrasomes are released by migrating cells and contain various RNAs and proteins, making them valuable for disease diagnosis through liquid biopsy. The researchers validated the WBFC method by analyzing migrasomes in cell culture medium, serum, and urine samples from kidney disease (KD) patients with podocyte injury and healthy volunteers. The results showed a significant increase in urinary podocyte-derived migrasome concentrations in KD patients compared to healthy individuals. Notably, these migrasomes expressed high levels of phospholipase A2 receptor (PLA2R) proteins, which could serve as a natural antigen for quantifying autoantibodies against PLA2R in the serum of patients with membranous nephropathy (MN). The study highlights the potential of urinary migrasomes as a promising biomarker for early diagnosis of KD with podocyte injury.