The article presents a method for the quantitative analysis of phospholipids using thin-layer chromatography (TLC). The method involves extracting lipids from tissues, preparing TLC plates, applying samples, and detecting phospholipid spots. The procedure allows for the separation and quantification of various phospholipid species, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, sphingomyelin, and lysophosphatidylcholine. The method is efficient, reproducible, and allows for the detection of phosphorus content in phospholipids. The results show that the major phospholipid components in rat liver are phosphatidylcholine (55.0%), phosphatidylethanolamine (25.3%), phosphatidylinositol (8.8%), phosphatidylserine (3.0%), sphingomyelin (1.8%), and lysophosphatidylcholine (0.9%). The method is simple, rapid, and reliable, making it suitable for the quantitative analysis of phospholipids in various tissues. The study also discusses the advantages of TLC over other methods, such as paper chromatography, and highlights the importance of phospholipid composition in biological systems. The method has been validated through recovery experiments and is applicable to the analysis of phospholipids in different biological samples.The article presents a method for the quantitative analysis of phospholipids using thin-layer chromatography (TLC). The method involves extracting lipids from tissues, preparing TLC plates, applying samples, and detecting phospholipid spots. The procedure allows for the separation and quantification of various phospholipid species, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, sphingomyelin, and lysophosphatidylcholine. The method is efficient, reproducible, and allows for the detection of phosphorus content in phospholipids. The results show that the major phospholipid components in rat liver are phosphatidylcholine (55.0%), phosphatidylethanolamine (25.3%), phosphatidylinositol (8.8%), phosphatidylserine (3.0%), sphingomyelin (1.8%), and lysophosphatidylcholine (0.9%). The method is simple, rapid, and reliable, making it suitable for the quantitative analysis of phospholipids in various tissues. The study also discusses the advantages of TLC over other methods, such as paper chromatography, and highlights the importance of phospholipid composition in biological systems. The method has been validated through recovery experiments and is applicable to the analysis of phospholipids in different biological samples.