The paper by V. P. Skipski, R. F. Peterson, and Marion Barclay presents a detailed method for the quantitative analysis of phospholipids using thin-layer chromatography (TLC). The authors describe the materials and methods used, including the preparation of TLC plates, sample application, chromatography, detection of spots, and phosphorus determination. They demonstrate the successful recovery of standard phospholipids and the separation of phospholipids from pooled rat liver. The results show that the major components are phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol, with lysophosphatidylcholine consistently present. The discussion highlights the advantages of TLC over other methods, such as its ability to handle larger amounts of starting material and provide clear separations without tailing. The method is also compared with previous techniques, showing improved efficiency in separating phosphatidylinositol and phosphatidylserine. The summary emphasizes the superior performance of the described method in terms of separation efficiency and versatility for further chemical analyses.The paper by V. P. Skipski, R. F. Peterson, and Marion Barclay presents a detailed method for the quantitative analysis of phospholipids using thin-layer chromatography (TLC). The authors describe the materials and methods used, including the preparation of TLC plates, sample application, chromatography, detection of spots, and phosphorus determination. They demonstrate the successful recovery of standard phospholipids and the separation of phospholipids from pooled rat liver. The results show that the major components are phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol, with lysophosphatidylcholine consistently present. The discussion highlights the advantages of TLC over other methods, such as its ability to handle larger amounts of starting material and provide clear separations without tailing. The method is also compared with previous techniques, showing improved efficiency in separating phosphatidylinositol and phosphatidylserine. The summary emphasizes the superior performance of the described method in terms of separation efficiency and versatility for further chemical analyses.