The article discusses the role of extracellular vesicles (EVs) in mammalian cells, focusing on their ability to deliver RNA and other biomolecules. EVs, which include exosomes, microvesicles, oncosomes, and apoptotic bodies, are released from various cell types and can carry a diverse range of cargo, such as RNAs, proteins, lipids, and DNA. These cargo molecules can be taken up by recipient cells, both locally and via biofluids, and elicit phenotypic responses. The RNA content of EVs, particularly small non-coding RNAs, has been shown to significantly influence the function and gene expression of recipient cells. The article highlights the potential of EVs as biomarkers and therapeutic vehicles, emphasizing the need for further research to understand their biogenesis, transport, and cargo delivery mechanisms. It also reviews the current state of knowledge about the packaging, transport, and function of RNAs in EVs, as well as the progress made in their clinical applications. The review covers various aspects, including the heterogeneity of EV populations, the mechanisms of RNA packaging, and the functional effects of EV-associated RNAs on recipient cells.The article discusses the role of extracellular vesicles (EVs) in mammalian cells, focusing on their ability to deliver RNA and other biomolecules. EVs, which include exosomes, microvesicles, oncosomes, and apoptotic bodies, are released from various cell types and can carry a diverse range of cargo, such as RNAs, proteins, lipids, and DNA. These cargo molecules can be taken up by recipient cells, both locally and via biofluids, and elicit phenotypic responses. The RNA content of EVs, particularly small non-coding RNAs, has been shown to significantly influence the function and gene expression of recipient cells. The article highlights the potential of EVs as biomarkers and therapeutic vehicles, emphasizing the need for further research to understand their biogenesis, transport, and cargo delivery mechanisms. It also reviews the current state of knowledge about the packaging, transport, and function of RNAs in EVs, as well as the progress made in their clinical applications. The review covers various aspects, including the heterogeneity of EV populations, the mechanisms of RNA packaging, and the functional effects of EV-associated RNAs on recipient cells.