The article discusses the effects of metallic surfaces on fluorescence, focusing on the concept of radiating plasmons (RPs) and how they influence the emission of fluorophores. Metallic particles and surfaces exhibit complex optical properties, including intense colors, surface plasmon resonance, and quenching of excited fluorophores. Recent studies have shown that interactions between fluorophores and metals can enhance fluorescence, develop assays based on fluorescence quenching, and produce directional radiation from fluorophores near thin metal films. The RP model explains these effects by considering the optical properties of metal structures calculated from electrodynamics, Mie theory, and Maxwell's equations.
The model suggests that small metal colloids quench fluorescence due to dominant absorption, while larger colloids enhance fluorescence due to dominant scattering. The ability of a metal's surface to absorb or reflect light is due to wavenumber matching requirements at the metal-sample interface. These considerations suggest that "lossy surface waves" which quench fluorescence are due to induced electron oscillations that cannot radiate to the far-field because wavevector matching is not possible. The energy from these waves can be recovered as emission by adjusting the sample to allow wavevector matching.
The article also discusses the effects of metallic surfaces on fluorescence, highlighting the long scientific history of these interactions. Studies have shown that fluorophores placed within wavelength-scale distances from a reflecting metallic surface result in oscillations of the emissive lifetime with distance from the metal surface. This effect can be explained by reflected far-field radiation from the fluorophore back on itself. However, at distances below 20 nm, the lifetime drops dramatically and the emission is strongly quenched, attributed to lossy surface waves (LSWs), dissipated losses, and similar terms.
The article explores the interactions of fluorophores with metallic particles and surfaces, finding that proximity of fluorophores within about 10 nm of silver island films (SIFs) results in increased emission intensities and decreased lifetimes. Similar enhancement effects are observed with silver colloids and fractal silver surfaces. The results are consistent with an increase in the radiative decay rate of the fluorophores, an unusual effect because the decay rate is determined by the extinction coefficient and the local refractive index.
Recent studies show that excited fluorophores near continuous thin silver films radiate into the underlying glass substrate with a sharp angular distribution, indicating that the radiation is from surface plasmons induced in the metal by the nearby excited fluorophores. This phenomenon is called surface-plasmon-coupled emission (SPCE). SPCE is also observed with gold and aluminum films. The results suggest that the excited fluorophores at these short distances induce electron oscillations in the metal film which radiate into the glass prism. This is surprising because the literature indicates that the emission is quenched at these short distances by lossy surface waves.
The article concludes that the radiating plasThe article discusses the effects of metallic surfaces on fluorescence, focusing on the concept of radiating plasmons (RPs) and how they influence the emission of fluorophores. Metallic particles and surfaces exhibit complex optical properties, including intense colors, surface plasmon resonance, and quenching of excited fluorophores. Recent studies have shown that interactions between fluorophores and metals can enhance fluorescence, develop assays based on fluorescence quenching, and produce directional radiation from fluorophores near thin metal films. The RP model explains these effects by considering the optical properties of metal structures calculated from electrodynamics, Mie theory, and Maxwell's equations.
The model suggests that small metal colloids quench fluorescence due to dominant absorption, while larger colloids enhance fluorescence due to dominant scattering. The ability of a metal's surface to absorb or reflect light is due to wavenumber matching requirements at the metal-sample interface. These considerations suggest that "lossy surface waves" which quench fluorescence are due to induced electron oscillations that cannot radiate to the far-field because wavevector matching is not possible. The energy from these waves can be recovered as emission by adjusting the sample to allow wavevector matching.
The article also discusses the effects of metallic surfaces on fluorescence, highlighting the long scientific history of these interactions. Studies have shown that fluorophores placed within wavelength-scale distances from a reflecting metallic surface result in oscillations of the emissive lifetime with distance from the metal surface. This effect can be explained by reflected far-field radiation from the fluorophore back on itself. However, at distances below 20 nm, the lifetime drops dramatically and the emission is strongly quenched, attributed to lossy surface waves (LSWs), dissipated losses, and similar terms.
The article explores the interactions of fluorophores with metallic particles and surfaces, finding that proximity of fluorophores within about 10 nm of silver island films (SIFs) results in increased emission intensities and decreased lifetimes. Similar enhancement effects are observed with silver colloids and fractal silver surfaces. The results are consistent with an increase in the radiative decay rate of the fluorophores, an unusual effect because the decay rate is determined by the extinction coefficient and the local refractive index.
Recent studies show that excited fluorophores near continuous thin silver films radiate into the underlying glass substrate with a sharp angular distribution, indicating that the radiation is from surface plasmons induced in the metal by the nearby excited fluorophores. This phenomenon is called surface-plasmon-coupled emission (SPCE). SPCE is also observed with gold and aluminum films. The results suggest that the excited fluorophores at these short distances induce electron oscillations in the metal film which radiate into the glass prism. This is surprising because the literature indicates that the emission is quenched at these short distances by lossy surface waves.
The article concludes that the radiating plas