Rapid biological synthesis of silver nanoparticles using plant leaf extracts

Rapid biological synthesis of silver nanoparticles using plant leaf extracts

26 April 2008 | Jae Yong Song · Beom Soo Kim
A rapid biological synthesis method for silver nanoparticles using plant leaf extracts was investigated. Five plant leaf extracts—pine, persimmon, ginkgo, magnolia, and platanus—were tested for their ability to reduce silver ions (Ag⁺) to metallic silver nanoparticles. Magnolia leaf broth was found to be the most effective, achieving over 90% conversion of Ag⁺ to Ag⁰ in just 11 minutes at 95°C. The synthesized nanoparticles were characterized using ICP, EDS, SEM, TEM, and particle analyzer, revealing average sizes between 15 and 500 nm. Particle size could be controlled by adjusting reaction temperature, leaf broth concentration, and AgNO₃ concentration. This method is environmentally friendly and offers synthesis rates comparable to chemical methods, making it suitable for applications in cosmetics, food, and medicine. Silver nanoparticles have unique properties due to their high surface-to-volume ratio and specific surface area, which enhance their antimicrobial activity. Silver has long been used for its antibacterial properties in medical and industrial applications. However, chemical methods for nanoparticle synthesis often involve toxic chemicals, prompting the development of eco-friendly alternatives. Biological methods using microorganisms, enzymes, or plant extracts have been proposed as alternatives. Plant-based methods are advantageous because they eliminate the need for complex cell culture maintenance and can be scaled up for large-scale production. This study compared the synthesis efficiency of different plant leaf extracts, focusing on the rate of silver nanoparticle formation and size control. The reaction was monitored using UV-vis spectroscopy, with the absorbance at 430 nm indicating nanoparticle concentration and conversion. The results showed that increasing reaction temperature improved synthesis rate and final conversion, while particle size decreased with higher temperatures. This study highlights the potential of plant-based methods for rapid, eco-friendly silver nanoparticle synthesis.A rapid biological synthesis method for silver nanoparticles using plant leaf extracts was investigated. Five plant leaf extracts—pine, persimmon, ginkgo, magnolia, and platanus—were tested for their ability to reduce silver ions (Ag⁺) to metallic silver nanoparticles. Magnolia leaf broth was found to be the most effective, achieving over 90% conversion of Ag⁺ to Ag⁰ in just 11 minutes at 95°C. The synthesized nanoparticles were characterized using ICP, EDS, SEM, TEM, and particle analyzer, revealing average sizes between 15 and 500 nm. Particle size could be controlled by adjusting reaction temperature, leaf broth concentration, and AgNO₃ concentration. This method is environmentally friendly and offers synthesis rates comparable to chemical methods, making it suitable for applications in cosmetics, food, and medicine. Silver nanoparticles have unique properties due to their high surface-to-volume ratio and specific surface area, which enhance their antimicrobial activity. Silver has long been used for its antibacterial properties in medical and industrial applications. However, chemical methods for nanoparticle synthesis often involve toxic chemicals, prompting the development of eco-friendly alternatives. Biological methods using microorganisms, enzymes, or plant extracts have been proposed as alternatives. Plant-based methods are advantageous because they eliminate the need for complex cell culture maintenance and can be scaled up for large-scale production. This study compared the synthesis efficiency of different plant leaf extracts, focusing on the rate of silver nanoparticle formation and size control. The reaction was monitored using UV-vis spectroscopy, with the absorbance at 430 nm indicating nanoparticle concentration and conversion. The results showed that increasing reaction temperature improved synthesis rate and final conversion, while particle size decreased with higher temperatures. This study highlights the potential of plant-based methods for rapid, eco-friendly silver nanoparticle synthesis.
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[slides and audio] Rapid biological synthesis of silver nanoparticles using plant leaf extracts