The article describes a novel real-time quantitative PCR method developed by Heid et al. This method uses a dual-labeled fluorogenic probe (TaqMan Probe) to measure PCR product accumulation, providing accurate and reproducible quantitation of gene copies. Unlike traditional PCR methods, real-time PCR does not require post-PCR sample handling, reducing the risk of contamination and increasing assay throughput. The method has a wide dynamic range, allowing for the measurement of target molecule concentrations over five orders of magnitude. The authors demonstrate the method's effectiveness by quantifying human factor VIII gene expression in transiently transfected 293 cells, showing that real-time PCR is highly reproducible and can be used for high-throughput screening assays in various applications, including gene expression analysis, gene copy assays, and genotyping. The method also offers greater specificity compared to intercalating dyes, as it does not detect primer dimers or nonspecific PCR products.The article describes a novel real-time quantitative PCR method developed by Heid et al. This method uses a dual-labeled fluorogenic probe (TaqMan Probe) to measure PCR product accumulation, providing accurate and reproducible quantitation of gene copies. Unlike traditional PCR methods, real-time PCR does not require post-PCR sample handling, reducing the risk of contamination and increasing assay throughput. The method has a wide dynamic range, allowing for the measurement of target molecule concentrations over five orders of magnitude. The authors demonstrate the method's effectiveness by quantifying human factor VIII gene expression in transiently transfected 293 cells, showing that real-time PCR is highly reproducible and can be used for high-throughput screening assays in various applications, including gene expression analysis, gene copy assays, and genotyping. The method also offers greater specificity compared to intercalating dyes, as it does not detect primer dimers or nonspecific PCR products.