This review article discusses recent advances in MXene-based electrochemical sensors (MECSens). MXene, a two-dimensional nanomaterial, is known for its high electrical conductivity, tunable structure, biocompatibility, and large surface area. These properties make it suitable for constructing MECSens with excellent electrochemical performance. The article highlights the importance of surface termination in enhancing the sensitivity and selectivity of MECSens. It also discusses the preparation and characterization of MECSens, including various synthesis methods such as F-containing etching, non-F-containing etching, and other techniques. The article covers different strategies for preparing MECSens, including dip-coating and printing methods, and discusses their advantages and drawbacks. The review also explores the applications of MECSens in detecting various analytes, including glucose, ascorbic acid, dopamine, uric acid, hydrogen peroxide, and heavy metal ions. The article emphasizes the potential of MECSens in clinical diagnosis, infectious disease surveillance, and food security. However, it also notes the challenges in long-term electrochemical analysis and the need for improvements in biocompatibility, stability, and selectivity. The review concludes with future perspectives on the development of MECSens for real-time health monitoring and in vivo electrochemical analysis.This review article discusses recent advances in MXene-based electrochemical sensors (MECSens). MXene, a two-dimensional nanomaterial, is known for its high electrical conductivity, tunable structure, biocompatibility, and large surface area. These properties make it suitable for constructing MECSens with excellent electrochemical performance. The article highlights the importance of surface termination in enhancing the sensitivity and selectivity of MECSens. It also discusses the preparation and characterization of MECSens, including various synthesis methods such as F-containing etching, non-F-containing etching, and other techniques. The article covers different strategies for preparing MECSens, including dip-coating and printing methods, and discusses their advantages and drawbacks. The review also explores the applications of MECSens in detecting various analytes, including glucose, ascorbic acid, dopamine, uric acid, hydrogen peroxide, and heavy metal ions. The article emphasizes the potential of MECSens in clinical diagnosis, infectious disease surveillance, and food security. However, it also notes the challenges in long-term electrochemical analysis and the need for improvements in biocompatibility, stability, and selectivity. The review concludes with future perspectives on the development of MECSens for real-time health monitoring and in vivo electrochemical analysis.