MXene, a family of two-dimensional (2D) nanomaterials, has emerged as a promising material for electrochemical sensors (MECSens) due to its excellent electrical conductivity, tunable structure, biocompatibility, and large surface area. This review provides an overview of the recent advances in MECSens based on MXene, highlighting their preparation methods, characterization techniques, and application scenarios. The preparation of MXene materials involves various etching methods, including F-containing etching, non-F-containing etching, and alkali etching, each with its advantages and drawbacks. MECSens are categorized into enzymatic and non-enzymatic types, with non-enzymatic sensors showing great potential for detecting glucose, ascorbic acid, dopamine, uric acid, hydrogen peroxide, and heavy metal ions. The review discusses the advantages and challenges of MECSens, emphasizing the need for improved antibiofouling ability, biocompatibility, and in vivo stability. Wearable and implantable sensors are identified as future research directions, with MXene's biocompatibility making it an ideal material for these applications. Overall, MXene-based MECSens have shown significant promise in various fields, but further improvements are required to address the limitations and enhance their performance for real-world applications.MXene, a family of two-dimensional (2D) nanomaterials, has emerged as a promising material for electrochemical sensors (MECSens) due to its excellent electrical conductivity, tunable structure, biocompatibility, and large surface area. This review provides an overview of the recent advances in MECSens based on MXene, highlighting their preparation methods, characterization techniques, and application scenarios. The preparation of MXene materials involves various etching methods, including F-containing etching, non-F-containing etching, and alkali etching, each with its advantages and drawbacks. MECSens are categorized into enzymatic and non-enzymatic types, with non-enzymatic sensors showing great potential for detecting glucose, ascorbic acid, dopamine, uric acid, hydrogen peroxide, and heavy metal ions. The review discusses the advantages and challenges of MECSens, emphasizing the need for improved antibiofouling ability, biocompatibility, and in vivo stability. Wearable and implantable sensors are identified as future research directions, with MXene's biocompatibility making it an ideal material for these applications. Overall, MXene-based MECSens have shown significant promise in various fields, but further improvements are required to address the limitations and enhance their performance for real-world applications.