The article reviews the synergistic effects of nanoparticles on biomedical applications, emphasizing their potential in drug delivery, bioimaging, tissue engineering, immunotherapy, and phototherapy. It discusses the morphological, size, surface area, ligand chemistry, and charge of nanoparticles, which influence their translocation and interaction with biological systems. The review highlights the enhanced properties of bimetallic nanoparticles (BMNPs) compared to monometallic nanoparticles (MNPs), including improved catalytic characteristics, optical, electrical, plasmonic, thermal, and magnetic properties. BMNPs are particularly useful in cancer diagnostics and therapy due to their ability to enhance multifunctional applications. The article also explores the green synthesis methods for BMNPs, which are environmentally friendly and biologically based, aligning with sustainability goals. Finally, it discusses the mechanisms of nanoparticle-cell interaction, including translocation, outer wrapping, embedding, and inner attachment, and their implications for biomedical applications.The article reviews the synergistic effects of nanoparticles on biomedical applications, emphasizing their potential in drug delivery, bioimaging, tissue engineering, immunotherapy, and phototherapy. It discusses the morphological, size, surface area, ligand chemistry, and charge of nanoparticles, which influence their translocation and interaction with biological systems. The review highlights the enhanced properties of bimetallic nanoparticles (BMNPs) compared to monometallic nanoparticles (MNPs), including improved catalytic characteristics, optical, electrical, plasmonic, thermal, and magnetic properties. BMNPs are particularly useful in cancer diagnostics and therapy due to their ability to enhance multifunctional applications. The article also explores the green synthesis methods for BMNPs, which are environmentally friendly and biologically based, aligning with sustainability goals. Finally, it discusses the mechanisms of nanoparticle-cell interaction, including translocation, outer wrapping, embedding, and inner attachment, and their implications for biomedical applications.