This review discusses recent advances in the synergistic effects of nanoparticles and their biomedical applications. The synergistic interaction between nanoparticles and biological systems is crucial for developing novel drug delivery systems with minimal toxicity. The review highlights the role of nanoparticle morphology, size, surface area, ligand chemistry, and charge in their translocation and interaction with cells and tissues. It also explores the potential of hybrid nanocarriers for biomedical applications such as bioimaging, tissue engineering, immunotherapy, and phototherapy. The review emphasizes the importance of understanding the bio/nano interface, including nanoparticle translocation, outer wrapping, embedding, and interior cellular attachment. The review also discusses the properties of metallic nanoparticles, including their surface plasmon resonance, catalytic activity, and potential applications in biomedical fields. It compares mono- and bimetallic nanoparticles, highlighting the advantages of bimetallic nanoparticles in terms of catalytic activity, optical properties, and functionality. The review explores the applications of gold nanoalloys in biomedical fields, including their use in drug delivery, biological imaging, and cancer therapy. It also discusses the potential of magnetic nanoalloys in hyperthermia ablation, magnetic tumor separation, and magnetic resonance imaging. The review further examines the mechanism of nanoparticle-cell interaction, including the different ways nanoparticles can interact with cell membranes, such as embedment, inner attachment, free translocation, and outer wrap. The review also discusses the green synthesis trends in bimetallic nanoparticles, emphasizing the importance of environmentally friendly methods in the synthesis of nanoparticles for biomedical applications. The review concludes that the synergistic effects of nanoparticles have significant potential in advancing green chemistry and sustainability, as required by the UN sustainability agenda. The review also highlights the need for further research on the interactions of nanoparticles with biological barriers and compartments to optimize internalization and site-specific drug release.This review discusses recent advances in the synergistic effects of nanoparticles and their biomedical applications. The synergistic interaction between nanoparticles and biological systems is crucial for developing novel drug delivery systems with minimal toxicity. The review highlights the role of nanoparticle morphology, size, surface area, ligand chemistry, and charge in their translocation and interaction with cells and tissues. It also explores the potential of hybrid nanocarriers for biomedical applications such as bioimaging, tissue engineering, immunotherapy, and phototherapy. The review emphasizes the importance of understanding the bio/nano interface, including nanoparticle translocation, outer wrapping, embedding, and interior cellular attachment. The review also discusses the properties of metallic nanoparticles, including their surface plasmon resonance, catalytic activity, and potential applications in biomedical fields. It compares mono- and bimetallic nanoparticles, highlighting the advantages of bimetallic nanoparticles in terms of catalytic activity, optical properties, and functionality. The review explores the applications of gold nanoalloys in biomedical fields, including their use in drug delivery, biological imaging, and cancer therapy. It also discusses the potential of magnetic nanoalloys in hyperthermia ablation, magnetic tumor separation, and magnetic resonance imaging. The review further examines the mechanism of nanoparticle-cell interaction, including the different ways nanoparticles can interact with cell membranes, such as embedment, inner attachment, free translocation, and outer wrap. The review also discusses the green synthesis trends in bimetallic nanoparticles, emphasizing the importance of environmentally friendly methods in the synthesis of nanoparticles for biomedical applications. The review concludes that the synergistic effects of nanoparticles have significant potential in advancing green chemistry and sustainability, as required by the UN sustainability agenda. The review also highlights the need for further research on the interactions of nanoparticles with biological barriers and compartments to optimize internalization and site-specific drug release.