Flavonoids are a class of polyphenolic compounds that play a crucial role in plant-microbe interactions, particularly in the rhizosphere. They are synthesized through various biosynthetic pathways and are involved in a wide range of biological activities, including inducing nodulation in legumes, forming mycorrhizal associations, and enhancing nutrient acquisition. Flavonoids are secreted into the rhizosphere through active and passive mechanisms and serve as signaling molecules that facilitate communication between plants and beneficial microbes. They also play a role in defense mechanisms against pathogens and in the regulation of microbial communities. Flavonoids have been shown to influence the establishment of symbiotic relationships with Rhizobium and arbuscular mycorrhizal fungi, aiding in the acquisition of essential nutrients such as phosphorus and iron. Additionally, flavonoids contribute to the protection of plants against biotic and abiotic stresses by modulating microbial interactions and enhancing plant defenses. The bioavailability and persistence of flavonoids in the rhizosphere are influenced by various factors, including soil composition, organic matter content, and microbial activity. Understanding the multifaceted roles of flavonoids in plant-microbe interactions is essential for developing strategies to enhance plant growth and resilience under various environmental conditions.Flavonoids are a class of polyphenolic compounds that play a crucial role in plant-microbe interactions, particularly in the rhizosphere. They are synthesized through various biosynthetic pathways and are involved in a wide range of biological activities, including inducing nodulation in legumes, forming mycorrhizal associations, and enhancing nutrient acquisition. Flavonoids are secreted into the rhizosphere through active and passive mechanisms and serve as signaling molecules that facilitate communication between plants and beneficial microbes. They also play a role in defense mechanisms against pathogens and in the regulation of microbial communities. Flavonoids have been shown to influence the establishment of symbiotic relationships with Rhizobium and arbuscular mycorrhizal fungi, aiding in the acquisition of essential nutrients such as phosphorus and iron. Additionally, flavonoids contribute to the protection of plants against biotic and abiotic stresses by modulating microbial interactions and enhancing plant defenses. The bioavailability and persistence of flavonoids in the rhizosphere are influenced by various factors, including soil composition, organic matter content, and microbial activity. Understanding the multifaceted roles of flavonoids in plant-microbe interactions is essential for developing strategies to enhance plant growth and resilience under various environmental conditions.