Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway have been reviewed, highlighting the complex regulatory mechanisms involving transcription factors from the R2R3-MYB, basic helix-loop-helix (bHLH), and WD40 classes. These factors form a ternary complex, the MYB-bHLH-WD40 (MBW) complex, which regulates genes involved in the biosynthesis of anthocyanins and condensed tannins. The MBW complex is essential for the spatial and temporal regulation of flavonoid biosynthesis, ensuring that these compounds are produced in the right tissues and at the appropriate developmental stages. Flavonoids are secondary metabolites with diverse roles in plant development, defense, and human health. They contribute to plant adaptation to environmental stresses and are important for human nutrition and medicine. The regulation of flavonoid biosynthesis is tightly controlled by transcription factors, with the MBW complex playing a central role. The bHLH, MYB, and WD40 proteins interact in a coordinated manner to activate or repress the expression of genes involved in the biosynthetic pathway. The bHLH proteins are involved in the regulation of various developmental processes, including trichome and root hair formation, and are part of the MBW complex that regulates flavonoid biosynthesis. The MYB proteins are responsible for recognizing specific DNA sequences and interacting with bHLH proteins to activate gene expression. The WD40 proteins act as a docking platform, facilitating interactions between the MYB and bHLH proteins and influencing the stability of the MBW complex. The regulation of the flavonoid biosynthetic pathway is highly specific, with different transcription factors involved in the regulation of different branches of the pathway. The MBW complex is essential for the regulation of anthocyanin biosynthesis, while other transcription factors regulate the biosynthesis of flavonols and condensed tannins. The complexity of the regulatory network is further highlighted by the autoregulation of the MBW complex, where the transcription factors regulate their own expression. Overall, the regulation of the flavonoid biosynthetic pathway is a highly coordinated process involving multiple transcription factors and their interactions. Understanding these regulatory mechanisms is crucial for the development of biotechnological tools to enhance the flavonoid content in plants, which has significant implications for agriculture and human health.Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway have been reviewed, highlighting the complex regulatory mechanisms involving transcription factors from the R2R3-MYB, basic helix-loop-helix (bHLH), and WD40 classes. These factors form a ternary complex, the MYB-bHLH-WD40 (MBW) complex, which regulates genes involved in the biosynthesis of anthocyanins and condensed tannins. The MBW complex is essential for the spatial and temporal regulation of flavonoid biosynthesis, ensuring that these compounds are produced in the right tissues and at the appropriate developmental stages. Flavonoids are secondary metabolites with diverse roles in plant development, defense, and human health. They contribute to plant adaptation to environmental stresses and are important for human nutrition and medicine. The regulation of flavonoid biosynthesis is tightly controlled by transcription factors, with the MBW complex playing a central role. The bHLH, MYB, and WD40 proteins interact in a coordinated manner to activate or repress the expression of genes involved in the biosynthetic pathway. The bHLH proteins are involved in the regulation of various developmental processes, including trichome and root hair formation, and are part of the MBW complex that regulates flavonoid biosynthesis. The MYB proteins are responsible for recognizing specific DNA sequences and interacting with bHLH proteins to activate gene expression. The WD40 proteins act as a docking platform, facilitating interactions between the MYB and bHLH proteins and influencing the stability of the MBW complex. The regulation of the flavonoid biosynthetic pathway is highly specific, with different transcription factors involved in the regulation of different branches of the pathway. The MBW complex is essential for the regulation of anthocyanin biosynthesis, while other transcription factors regulate the biosynthesis of flavonols and condensed tannins. The complexity of the regulatory network is further highlighted by the autoregulation of the MBW complex, where the transcription factors regulate their own expression. Overall, the regulation of the flavonoid biosynthetic pathway is a highly coordinated process involving multiple transcription factors and their interactions. Understanding these regulatory mechanisms is crucial for the development of biotechnological tools to enhance the flavonoid content in plants, which has significant implications for agriculture and human health.