Reconfigurable flexible metasurfaces have shown great potential in biomedical applications. This review summarizes recent advances in the design and fabrication of stretchable reconfigurable metasurfaces and their applications. Metasurfaces, two-dimensional structures with unique optical properties, offer mechanical flexibility and are ideal for wearable devices. Recent research has focused on developing reconfigurable metasurfaces that can dynamically adjust their properties, enabling applications in bioimaging, immunoassay, and cancer detection. Various tuning mechanisms, including MEMS, origami/kirigami, photoactuation, electroactuation, and magnetoactuation, have been explored to achieve flexibility, stretchability, and reconfigurability. These mechanisms allow for precise control of metasurface properties, enabling functions such as tunable optical devices, sensors, and detectors. The fabrication of flexible and stretchable metasurfaces involves advanced techniques like nanoimprint lithography, 3D printing, and soft lithography. These techniques enable the creation of complex structures on flexible substrates, such as PDMS and PET. Applications of reconfigurable metasurfaces include bioimaging, where they can provide high-resolution imaging with minimal harm to the body, and immunoassay, where they enhance sensitivity and accuracy in detecting biomarkers. The integration of machine learning with metasurfaces has further improved the efficiency of bioimaging and data processing. Overall, reconfigurable flexible metasurfaces represent a promising direction for future biomedical applications, offering enhanced flexibility, reconfigurability, and functionality.Reconfigurable flexible metasurfaces have shown great potential in biomedical applications. This review summarizes recent advances in the design and fabrication of stretchable reconfigurable metasurfaces and their applications. Metasurfaces, two-dimensional structures with unique optical properties, offer mechanical flexibility and are ideal for wearable devices. Recent research has focused on developing reconfigurable metasurfaces that can dynamically adjust their properties, enabling applications in bioimaging, immunoassay, and cancer detection. Various tuning mechanisms, including MEMS, origami/kirigami, photoactuation, electroactuation, and magnetoactuation, have been explored to achieve flexibility, stretchability, and reconfigurability. These mechanisms allow for precise control of metasurface properties, enabling functions such as tunable optical devices, sensors, and detectors. The fabrication of flexible and stretchable metasurfaces involves advanced techniques like nanoimprint lithography, 3D printing, and soft lithography. These techniques enable the creation of complex structures on flexible substrates, such as PDMS and PET. Applications of reconfigurable metasurfaces include bioimaging, where they can provide high-resolution imaging with minimal harm to the body, and immunoassay, where they enhance sensitivity and accuracy in detecting biomarkers. The integration of machine learning with metasurfaces has further improved the efficiency of bioimaging and data processing. Overall, reconfigurable flexible metasurfaces represent a promising direction for future biomedical applications, offering enhanced flexibility, reconfigurability, and functionality.