The article by Kim D. Pruitt and Donna R. Maglott from the National Center for Biotechnology Information (NCBI) introduces two key resources, LocusLink and RefSeq, which are essential for gene-centered research. LocusLink provides a comprehensive directory of gene information, including nomenclature, database identifiers, disease associations, map positions, and sequence accessions, for key genomes such as fruit fly, human, mouse, rat, and zebrafish. RefSeq offers reference sequence standards for genomes, transcripts, and proteins, with a focus on human, mouse, and rat mRNA RefSeqs and their corresponding proteins. These resources are continuously updated and linked to other NCBI databases, facilitating navigation and enhancing discovery opportunities. The article also highlights new features and expanded functionalities in both LocusLink and RefSeq, including community-generated functional annotations, homologous sequence pairs, and detailed sequence information. The growth and maintenance of these resources are discussed, emphasizing their role in supporting research on genes, gene families, variation, gene expression, and genome annotation.The article by Kim D. Pruitt and Donna R. Maglott from the National Center for Biotechnology Information (NCBI) introduces two key resources, LocusLink and RefSeq, which are essential for gene-centered research. LocusLink provides a comprehensive directory of gene information, including nomenclature, database identifiers, disease associations, map positions, and sequence accessions, for key genomes such as fruit fly, human, mouse, rat, and zebrafish. RefSeq offers reference sequence standards for genomes, transcripts, and proteins, with a focus on human, mouse, and rat mRNA RefSeqs and their corresponding proteins. These resources are continuously updated and linked to other NCBI databases, facilitating navigation and enhancing discovery opportunities. The article also highlights new features and expanded functionalities in both LocusLink and RefSeq, including community-generated functional annotations, homologous sequence pairs, and detailed sequence information. The growth and maintenance of these resources are discussed, emphasizing their role in supporting research on genes, gene families, variation, gene expression, and genome annotation.