The article reviews the role of RNA-binding proteins (RBPs) in the biogenesis of microRNAs (miRNAs). MiRNAs are small non-coding RNAs that regulate gene expression through post-transcriptional mechanisms. The biogenesis of miRNAs involves several steps, including the processing of primary miRNA (pri-miRNA) to precursor miRNA (pre-miRNA) by the microprocessor complex (Drosha and Dicer), nuclear export of pre-miRNA by exportin 5, and maturation of pre-miRNA to mature miRNA by Dicer. RBPs play a crucial role in these processes by interacting with miRNA precursors and mature miRNAs. These interactions help fine-tune miRNA expression and function. The article highlights the specific RBPs involved in each step of miRNA biogenesis, such as hnRNPA1, TDP-43, KSRP, and LIN28B, which influence the efficiency and outcome of miRNA processing. Additionally, the article discusses non-canonical miRNA biogenesis pathways, including mirtron and modified miRNA biogenesis, and the cluster assistance phenomenon, where the presence of neighboring miRNAs can enhance the processing of sub-optimal pri-miRNAs. The authors conclude by emphasizing the importance of RBPs in maintaining miRNA expression and function, and the need for further research to fully understand their roles in miRNA biogenesis.The article reviews the role of RNA-binding proteins (RBPs) in the biogenesis of microRNAs (miRNAs). MiRNAs are small non-coding RNAs that regulate gene expression through post-transcriptional mechanisms. The biogenesis of miRNAs involves several steps, including the processing of primary miRNA (pri-miRNA) to precursor miRNA (pre-miRNA) by the microprocessor complex (Drosha and Dicer), nuclear export of pre-miRNA by exportin 5, and maturation of pre-miRNA to mature miRNA by Dicer. RBPs play a crucial role in these processes by interacting with miRNA precursors and mature miRNAs. These interactions help fine-tune miRNA expression and function. The article highlights the specific RBPs involved in each step of miRNA biogenesis, such as hnRNPA1, TDP-43, KSRP, and LIN28B, which influence the efficiency and outcome of miRNA processing. Additionally, the article discusses non-canonical miRNA biogenesis pathways, including mirtron and modified miRNA biogenesis, and the cluster assistance phenomenon, where the presence of neighboring miRNAs can enhance the processing of sub-optimal pri-miRNAs. The authors conclude by emphasizing the importance of RBPs in maintaining miRNA expression and function, and the need for further research to fully understand their roles in miRNA biogenesis.