This study evaluated two non-conventional extraction techniques—microwave hydrodiffusion and gravity (MHG) and pressurized-liquid extraction (PLE)—for obtaining bioactive compounds from *Solanum viarum*. The aim was to assess the yield and chemical composition of extracts using these methods. For PLE, the percentage of ethanol and temperature were optimized, while for MHG, temperature and pressure were evaluated. PLE yielded higher extract yields (up to 26.11% for fruit and up to 11.22% for root) and a higher concentration of chemical compounds compared to MHG. Both techniques effectively extracted bioactive compounds such as integerrimine, senecionine, and quinic acid. The study highlights the potential of innovative extraction technologies for unlocking the bioactive potential of Brazilian biodiversity, suggesting further research on the optimization and application of these methods.This study evaluated two non-conventional extraction techniques—microwave hydrodiffusion and gravity (MHG) and pressurized-liquid extraction (PLE)—for obtaining bioactive compounds from *Solanum viarum*. The aim was to assess the yield and chemical composition of extracts using these methods. For PLE, the percentage of ethanol and temperature were optimized, while for MHG, temperature and pressure were evaluated. PLE yielded higher extract yields (up to 26.11% for fruit and up to 11.22% for root) and a higher concentration of chemical compounds compared to MHG. Both techniques effectively extracted bioactive compounds such as integerrimine, senecionine, and quinic acid. The study highlights the potential of innovative extraction technologies for unlocking the bioactive potential of Brazilian biodiversity, suggesting further research on the optimization and application of these methods.