This review summarizes recent advances in electrospun nanofiber membranes for non-invasive medical applications. Electrospinning is a versatile technique for producing nanofibers with high surface area, tunable pore structures, and excellent mechanical and biocompatibility properties. These nanofibers have shown great potential in various medical fields, including drug delivery, tissue engineering, biosensing, and wearable electronics. The review discusses different electrospinning methods, such as needle-based and needleless electrospinning, and highlights the structural diversity of nanofibers, including homogeneous, beaded, core-shell, ribbon, and porous fibers. The review also explores the applications of electrospun nanofibers in non-invasive medical fields, such as health monitoring, personal protective gear, thermal regulation, and wound care. It emphasizes the importance of electrospun nanofibers in improving human health and discusses the challenges and future directions of this technology. The review also covers the application of electrospun nanofibers in wearable sensors for monitoring electrophysiological signals, human movement, temperature, respiratory humidity, and biosensors for monitoring glucose, uric acid, pH, and inorganic substances. The review concludes that electrospun nanofibers have significant potential in non-invasive medical applications and require further research to overcome existing challenges and enhance their performance.This review summarizes recent advances in electrospun nanofiber membranes for non-invasive medical applications. Electrospinning is a versatile technique for producing nanofibers with high surface area, tunable pore structures, and excellent mechanical and biocompatibility properties. These nanofibers have shown great potential in various medical fields, including drug delivery, tissue engineering, biosensing, and wearable electronics. The review discusses different electrospinning methods, such as needle-based and needleless electrospinning, and highlights the structural diversity of nanofibers, including homogeneous, beaded, core-shell, ribbon, and porous fibers. The review also explores the applications of electrospun nanofibers in non-invasive medical fields, such as health monitoring, personal protective gear, thermal regulation, and wound care. It emphasizes the importance of electrospun nanofibers in improving human health and discusses the challenges and future directions of this technology. The review also covers the application of electrospun nanofibers in wearable sensors for monitoring electrophysiological signals, human movement, temperature, respiratory humidity, and biosensors for monitoring glucose, uric acid, pH, and inorganic substances. The review concludes that electrospun nanofibers have significant potential in non-invasive medical applications and require further research to overcome existing challenges and enhance their performance.