The paper provides a comprehensive overview of the applications of electrospun nanofibers in non-invasive medical fields, highlighting their potential in improving human health. Electrospinning, an efficient nanofiber fabrication process, is characterized by its ability to produce nanofibers with high specific surface area, tunable pore structures, and excellent biocompatibility. The review covers the production techniques of electrospun nanofibers, including needle-based and needleless methods, and discusses the structural diversity of these fibers, such as homogeneous, beaded, core-shell, ribbon, and porous fibers. The applications of electrospun nanofibers in wearable sensors and personal protective equipment (PPE) are detailed. In wearable sensors, electrospun nanofibers are used for monitoring electrophysiological signals (ECG, EMG, EEG), human movement, temperature, and respiratory humidity. These sensors offer advantages such as breathability, biocompatibility, flexibility, and high sensitivity. In PPE, electrospun nanofibers are used in masks, providing efficient particulate filtration, antibacterial and antiviral capabilities, and enhanced comfort. The paper also addresses the challenges and future directions in the field, emphasizing the need for further research to improve the performance and widespread adoption of electrospun nanofiber technology in non-invasive medicine and healthcare.The paper provides a comprehensive overview of the applications of electrospun nanofibers in non-invasive medical fields, highlighting their potential in improving human health. Electrospinning, an efficient nanofiber fabrication process, is characterized by its ability to produce nanofibers with high specific surface area, tunable pore structures, and excellent biocompatibility. The review covers the production techniques of electrospun nanofibers, including needle-based and needleless methods, and discusses the structural diversity of these fibers, such as homogeneous, beaded, core-shell, ribbon, and porous fibers. The applications of electrospun nanofibers in wearable sensors and personal protective equipment (PPE) are detailed. In wearable sensors, electrospun nanofibers are used for monitoring electrophysiological signals (ECG, EMG, EEG), human movement, temperature, and respiratory humidity. These sensors offer advantages such as breathability, biocompatibility, flexibility, and high sensitivity. In PPE, electrospun nanofibers are used in masks, providing efficient particulate filtration, antibacterial and antiviral capabilities, and enhanced comfort. The paper also addresses the challenges and future directions in the field, emphasizing the need for further research to improve the performance and widespread adoption of electrospun nanofiber technology in non-invasive medicine and healthcare.