This article, authored by Albert B. Sabin, provides a comprehensive overview of the research on dengue fever conducted during World War II by the U.S. Army. Prior to the war, significant contributions were made by U.S. Army medical personnel, including evidence of the viral etiology of dengue and the period of infectivity of patients for Aedes aegypti mosquitoes. However, by the start of World War II, there was a lack of systematic studies due to the absence of suitable strains, laboratory animals, and methods for in vitro cultivation. The primary objectives of the research were to develop an immunizing agent against dengue and a practical diagnostic test to identify the role of dengue in "fevers of unknown origin." The research was organized in a dengue-free area of the United States, with human volunteers located nearby. The study involved recovering dengue virus strains from patients in Hawaii, New Guinea, and India, and testing them in human volunteers. Key findings included the identification of different strains of dengue virus, the development of a mouse-adapted strain that produced a modified, less severe form of the disease, and the demonstration of type-specific neutralizing antibodies. The research also explored the interference between dengue and yellow fever viruses, and the potential for using mouse-adapted dengue virus as a vaccine. The article concludes with discussions on the infectivity of Aedes aegypti mosquitoes feeding on vaccinated individuals and the cultivation of mouse-adapted dengue virus in chick embryos. The research laid the foundation for future studies and the development of防控措施 against dengue fever.This article, authored by Albert B. Sabin, provides a comprehensive overview of the research on dengue fever conducted during World War II by the U.S. Army. Prior to the war, significant contributions were made by U.S. Army medical personnel, including evidence of the viral etiology of dengue and the period of infectivity of patients for Aedes aegypti mosquitoes. However, by the start of World War II, there was a lack of systematic studies due to the absence of suitable strains, laboratory animals, and methods for in vitro cultivation. The primary objectives of the research were to develop an immunizing agent against dengue and a practical diagnostic test to identify the role of dengue in "fevers of unknown origin." The research was organized in a dengue-free area of the United States, with human volunteers located nearby. The study involved recovering dengue virus strains from patients in Hawaii, New Guinea, and India, and testing them in human volunteers. Key findings included the identification of different strains of dengue virus, the development of a mouse-adapted strain that produced a modified, less severe form of the disease, and the demonstration of type-specific neutralizing antibodies. The research also explored the interference between dengue and yellow fever viruses, and the potential for using mouse-adapted dengue virus as a vaccine. The article concludes with discussions on the infectivity of Aedes aegypti mosquitoes feeding on vaccinated individuals and the cultivation of mouse-adapted dengue virus in chick embryos. The research laid the foundation for future studies and the development of防控措施 against dengue fever.