N1-methyladenosine (m1A) is a post-transcriptionally modified RNA molecule that plays a crucial role in regulating various biological functions, particularly in cancer cell invasion, proliferation, and cell cycle regulation. Recent years have seen a growing interest in the study of m1A modification in cancer. This review provides a comprehensive overview of the methodologies used to detect m1A modification, including traditional techniques like 2D-TLC, HPLC, and LC-MS, as well as high-throughput sequencing methods such as ARM-seq, m1A-ID-seq, MeRIP-seq, and m1A-MAP-seq. These methods have evolved from chemical analysis to high-resolution sequencing, enhancing the accuracy and sensitivity of m1A detection. The review also delves into the key players in m1A modification, known as "writers," "erasers," and "readers." Writers, such as TRMT6, TRMT6A, TRMT6B, TRMT10C, NML, and ALKBH1, ALKBH3, and FTO, are responsible for the methylation of m1A. Erasers, including ALKBH1, ALKBH3, and FTO, remove m1A modifications. Readers, such as YTHDF1, YTHDF2, YTHDF3, and YTHDC1, recognize and bind to m1A-modified RNA. The intricate relationship between m1A modification and its regulators is explored, highlighting their implications for cancer development and progression. The review further discusses the potential of m1A modification in cancer diagnosis, treatment, and prognosis. It highlights how m1A modification can facilitate the discovery of novel approaches for cancer management. The detection methods and regulatory mechanisms of m1A in various cancers are summarized, providing valuable insights for future research and clinical applications. Keywords: N1-methyladenosine, Detect method, Writers, Erasers, Readers, Regulation of cancerN1-methyladenosine (m1A) is a post-transcriptionally modified RNA molecule that plays a crucial role in regulating various biological functions, particularly in cancer cell invasion, proliferation, and cell cycle regulation. Recent years have seen a growing interest in the study of m1A modification in cancer. This review provides a comprehensive overview of the methodologies used to detect m1A modification, including traditional techniques like 2D-TLC, HPLC, and LC-MS, as well as high-throughput sequencing methods such as ARM-seq, m1A-ID-seq, MeRIP-seq, and m1A-MAP-seq. These methods have evolved from chemical analysis to high-resolution sequencing, enhancing the accuracy and sensitivity of m1A detection. The review also delves into the key players in m1A modification, known as "writers," "erasers," and "readers." Writers, such as TRMT6, TRMT6A, TRMT6B, TRMT10C, NML, and ALKBH1, ALKBH3, and FTO, are responsible for the methylation of m1A. Erasers, including ALKBH1, ALKBH3, and FTO, remove m1A modifications. Readers, such as YTHDF1, YTHDF2, YTHDF3, and YTHDC1, recognize and bind to m1A-modified RNA. The intricate relationship between m1A modification and its regulators is explored, highlighting their implications for cancer development and progression. The review further discusses the potential of m1A modification in cancer diagnosis, treatment, and prognosis. It highlights how m1A modification can facilitate the discovery of novel approaches for cancer management. The detection methods and regulatory mechanisms of m1A in various cancers are summarized, providing valuable insights for future research and clinical applications. Keywords: N1-methyladenosine, Detect method, Writers, Erasers, Readers, Regulation of cancer