Luteolin, a natural flavone found in various fruits and vegetables, has emerged as a promising chemopreventive agent against several types of cancer. Its anticancer properties are attributed to its ability to induce apoptosis, arrest the cell cycle, inhibit angiogenesis, and reduce metastasis and cell proliferation. Luteolin's mechanisms of action involve engaging multiple molecular targets and modifying signaling pathways in tumor cells. It has been shown to slow the progression of breast, colorectal, lung, prostate, liver, skin, pancreatic, oral, and gastric cancers. Additionally, luteolin exhibits antioxidant properties and can be used to prevent adverse reactions associated with Doxorubicin (DOX) chemotherapy. It also shows potential as a synergistic agent to overcome drug resistance in cancer cells. This review covers recent literature on luteolin's anticancer potential, its mechanisms of action, and its role as a complementary medicine for cancer prevention and treatment. The biosynthesis of luteolin and its toxicological profiles are also discussed, highlighting the need for further research to optimize its therapeutic applications.Luteolin, a natural flavone found in various fruits and vegetables, has emerged as a promising chemopreventive agent against several types of cancer. Its anticancer properties are attributed to its ability to induce apoptosis, arrest the cell cycle, inhibit angiogenesis, and reduce metastasis and cell proliferation. Luteolin's mechanisms of action involve engaging multiple molecular targets and modifying signaling pathways in tumor cells. It has been shown to slow the progression of breast, colorectal, lung, prostate, liver, skin, pancreatic, oral, and gastric cancers. Additionally, luteolin exhibits antioxidant properties and can be used to prevent adverse reactions associated with Doxorubicin (DOX) chemotherapy. It also shows potential as a synergistic agent to overcome drug resistance in cancer cells. This review covers recent literature on luteolin's anticancer potential, its mechanisms of action, and its role as a complementary medicine for cancer prevention and treatment. The biosynthesis of luteolin and its toxicological profiles are also discussed, highlighting the need for further research to optimize its therapeutic applications.