The article introduces SYRCLE's Risk of Bias (RoB) tool for animal intervention studies, which is adapted from the Cochrane RoB tool. The tool aims to assess the methodological quality of animal studies by addressing specific biases that are relevant to this field. The development process involved comparing the differences between randomized clinical trials (RCTs) and animal studies, formulating signaling questions, and conducting inter-observer agreement studies. The resulting tool includes 10 entries related to selection bias, performance bias, detection bias, attrition bias, reporting bias, and other biases. The tool is designed to enhance transparency and applicability, and its widespread adoption is expected to improve the critical appraisal of evidence from animal studies, facilitating the translation of animal research into clinical practice.The article introduces SYRCLE's Risk of Bias (RoB) tool for animal intervention studies, which is adapted from the Cochrane RoB tool. The tool aims to assess the methodological quality of animal studies by addressing specific biases that are relevant to this field. The development process involved comparing the differences between randomized clinical trials (RCTs) and animal studies, formulating signaling questions, and conducting inter-observer agreement studies. The resulting tool includes 10 entries related to selection bias, performance bias, detection bias, attrition bias, reporting bias, and other biases. The tool is designed to enhance transparency and applicability, and its widespread adoption is expected to improve the critical appraisal of evidence from animal studies, facilitating the translation of animal research into clinical practice.