This paper describes a method for self-assembling DNA into nanoscale three-dimensional shapes. The researchers developed a technique that uses a scaffold strand folded into a flat array of antiparallel helices, with hundreds of oligonucleotide staple strands interacting with the scaffold to form custom three-dimensional shapes. The method allows for the design and assembly of complex nanostructures, including six different shapes with precisely controlled dimensions ranging from 10 to 100 nm. The shapes include a monolith, square nut, railed bridge, genie bottle, stacked cross, and slotted cross. The study also demonstrates hierarchical assembly of structures such as homomultimeric linear tracks and heterotrimeric wireframe icosahedra. The design process is analogous to sculpting from a porous crystalline block, where the block is a honeycomb lattice of antiparallel scaffold helices. Complementary staple strands wind around the scaffold strands to assemble B-form double helices with specific geometrical parameters. Crossovers between adjacent staple and scaffold helices are restricted to specific positions, and the design process involves carving away duplex segments to define the target shape and introducing scaffold crossovers to create a singular scaffold path that visits all remaining duplex segments. The study also discusses the importance of proper folding conditions, including the duration of thermal ramp, divalent-cation concentration, and monovalent-cation concentration. The researchers found that higher concentrations of MgCl2 and lower concentrations of NaCl led to better folding quality. The study also highlights the use of the caDNAno software for designing DNA origami structures, which allows for the rapid generation of staple sequences for new shapes. The study demonstrates that the honeycomb-pleated origami approach can be used to approximate various three-dimensional shapes, including the monolith, square nut, railed bridge, genie bottle, stacked cross, and slotted cross. The results show that the method is effective in creating complex nanostructures with precise dimensions and shapes. The study also discusses the potential applications of this method in creating sophisticated devices with nanometer-scale features. The researchers conclude that this method provides a general route to the manufacture of such devices.This paper describes a method for self-assembling DNA into nanoscale three-dimensional shapes. The researchers developed a technique that uses a scaffold strand folded into a flat array of antiparallel helices, with hundreds of oligonucleotide staple strands interacting with the scaffold to form custom three-dimensional shapes. The method allows for the design and assembly of complex nanostructures, including six different shapes with precisely controlled dimensions ranging from 10 to 100 nm. The shapes include a monolith, square nut, railed bridge, genie bottle, stacked cross, and slotted cross. The study also demonstrates hierarchical assembly of structures such as homomultimeric linear tracks and heterotrimeric wireframe icosahedra. The design process is analogous to sculpting from a porous crystalline block, where the block is a honeycomb lattice of antiparallel scaffold helices. Complementary staple strands wind around the scaffold strands to assemble B-form double helices with specific geometrical parameters. Crossovers between adjacent staple and scaffold helices are restricted to specific positions, and the design process involves carving away duplex segments to define the target shape and introducing scaffold crossovers to create a singular scaffold path that visits all remaining duplex segments. The study also discusses the importance of proper folding conditions, including the duration of thermal ramp, divalent-cation concentration, and monovalent-cation concentration. The researchers found that higher concentrations of MgCl2 and lower concentrations of NaCl led to better folding quality. The study also highlights the use of the caDNAno software for designing DNA origami structures, which allows for the rapid generation of staple sequences for new shapes. The study demonstrates that the honeycomb-pleated origami approach can be used to approximate various three-dimensional shapes, including the monolith, square nut, railed bridge, genie bottle, stacked cross, and slotted cross. The results show that the method is effective in creating complex nanostructures with precise dimensions and shapes. The study also discusses the potential applications of this method in creating sophisticated devices with nanometer-scale features. The researchers conclude that this method provides a general route to the manufacture of such devices.