This review article by Zhang et al. provides a comprehensive overview of silver nanoparticles (AgNPs) and their applications in various fields, particularly in cancer therapy. The authors discuss the synthesis methods of AgNPs, including physical, chemical, and biological approaches, emphasizing the advantages and limitations of each method. They highlight the importance of characterizing AgNPs using techniques such as UV-vis spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS), and transmission electron microscopy (TEM) to understand their properties and ensure safety and efficacy. The article also delves into the multifunctional bio-applications of AgNPs, including their antibacterial, antifungal, antiviral, anti-inflammatory, and anti-angiogenic properties. It reviews the mechanisms by which AgNPs exert these effects, such as disrupting bacterial cell membranes, inhibiting fungal growth, and modulating inflammatory responses. Additionally, the review explores the therapeutic potential of AgNPs in cancer treatment, discussing their ability to enhance the effectiveness of anticancer drugs and their potential as targeted agents. Finally, the authors address the challenges and future perspectives of using AgNPs in cancer therapy, emphasizing the need for further research to optimize their use in clinical settings. The review concludes by highlighting the promising future of AgNPs in nanomedicine and their potential to revolutionize disease diagnosis and treatment.This review article by Zhang et al. provides a comprehensive overview of silver nanoparticles (AgNPs) and their applications in various fields, particularly in cancer therapy. The authors discuss the synthesis methods of AgNPs, including physical, chemical, and biological approaches, emphasizing the advantages and limitations of each method. They highlight the importance of characterizing AgNPs using techniques such as UV-vis spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS), and transmission electron microscopy (TEM) to understand their properties and ensure safety and efficacy. The article also delves into the multifunctional bio-applications of AgNPs, including their antibacterial, antifungal, antiviral, anti-inflammatory, and anti-angiogenic properties. It reviews the mechanisms by which AgNPs exert these effects, such as disrupting bacterial cell membranes, inhibiting fungal growth, and modulating inflammatory responses. Additionally, the review explores the therapeutic potential of AgNPs in cancer treatment, discussing their ability to enhance the effectiveness of anticancer drugs and their potential as targeted agents. Finally, the authors address the challenges and future perspectives of using AgNPs in cancer therapy, emphasizing the need for further research to optimize their use in clinical settings. The review concludes by highlighting the promising future of AgNPs in nanomedicine and their potential to revolutionize disease diagnosis and treatment.