The article presents a simplified approach to the identification of aerobic actinomycetes using thin-layer chromatography (TLC). The method involves analyzing whole cells for diaminopimelic acid (DAP) and carbohydrates, as well as testing the ability of the organisms to decompose casein, tyrosine, and xanthine media. The authors describe a thin-layer chromatographic procedure that is easier to perform and more efficient than traditional paper chromatography. The system was successfully applied to identify 18 reference cultures and group 35 clinical isolates. The method is suitable for routine clinical laboratories, providing rapid and accurate identification of aerobic actinomycetes, particularly *Nocardia*, which are increasingly reported in clinical settings. The article also discusses the limitations and considerations of the method, emphasizing its utility in diagnosing infections caused by these organisms.The article presents a simplified approach to the identification of aerobic actinomycetes using thin-layer chromatography (TLC). The method involves analyzing whole cells for diaminopimelic acid (DAP) and carbohydrates, as well as testing the ability of the organisms to decompose casein, tyrosine, and xanthine media. The authors describe a thin-layer chromatographic procedure that is easier to perform and more efficient than traditional paper chromatography. The system was successfully applied to identify 18 reference cultures and group 35 clinical isolates. The method is suitable for routine clinical laboratories, providing rapid and accurate identification of aerobic actinomycetes, particularly *Nocardia*, which are increasingly reported in clinical settings. The article also discusses the limitations and considerations of the method, emphasizing its utility in diagnosing infections caused by these organisms.