A new non-enzymatic glucose sensing platform is developed using a single-component electroactive polymer, EDOT-PBA. This polymer integrates both conducting and receptor functionalities in a single organic component, eliminating the need for complex composite materials. Two polymer film architectures, pristine PEDOT-PBA (NIP) and molecularly imprinted PEDOT-PBA (MIP), are fabricated through electrodeposition. Both architectures demonstrate effective glucose binding and signal transduction. The MIP architecture shows faster stabilization upon glucose uptake, lower limit of detection, lower standard deviation, and a broader linear range compared to the NIP. The MIP also exhibits better reproducibility and sensitivity. The polymer is used as a gate electrode in organic electrochemical transistors (OECTs), enabling efficient glucose sensing. The MIP provides a more stable and sensitive response, with a lower limit of detection of 22.3 μM and a linear range from 10 μM to 10 mM. The sensor is tested in various conditions, including different ionic strengths, pH levels, and temperatures, showing good stability and performance. The MIP's design allows for selective sensing of glucose, with minimal interference from other molecules. The platform offers a versatile and adaptable approach for developing sensors for a wide range of target molecules. The study highlights the potential of single-component electroactive polymers for non-enzymatic glucose sensing and other applications in organic bioelectronics.A new non-enzymatic glucose sensing platform is developed using a single-component electroactive polymer, EDOT-PBA. This polymer integrates both conducting and receptor functionalities in a single organic component, eliminating the need for complex composite materials. Two polymer film architectures, pristine PEDOT-PBA (NIP) and molecularly imprinted PEDOT-PBA (MIP), are fabricated through electrodeposition. Both architectures demonstrate effective glucose binding and signal transduction. The MIP architecture shows faster stabilization upon glucose uptake, lower limit of detection, lower standard deviation, and a broader linear range compared to the NIP. The MIP also exhibits better reproducibility and sensitivity. The polymer is used as a gate electrode in organic electrochemical transistors (OECTs), enabling efficient glucose sensing. The MIP provides a more stable and sensitive response, with a lower limit of detection of 22.3 μM and a linear range from 10 μM to 10 mM. The sensor is tested in various conditions, including different ionic strengths, pH levels, and temperatures, showing good stability and performance. The MIP's design allows for selective sensing of glucose, with minimal interference from other molecules. The platform offers a versatile and adaptable approach for developing sensors for a wide range of target molecules. The study highlights the potential of single-component electroactive polymers for non-enzymatic glucose sensing and other applications in organic bioelectronics.