This study presents a non-enzymatic glucose sensing platform using a newly synthesized functionalized monomer, EDOT-PBA, which integrates electrically conducting and receptor moieties within a single organic component. The electrodeposition technique was used to produce two distinct polymer film architectures: pristine PEDOT-PBA (NIP) and molecularly imprinted PEDOT-PBA (MIP). Both architectures demonstrated proficient glucose binding and signal transduction capabilities. Notably, the MIP architecture showed faster stabilization upon glucose uptake, a lower limit of detection, a lower standard deviation, and a broader linear response range compared to the NIP. The MIP architecture's advantages include enhanced film uniformity, improved reproducibility, and a more straightforward analysis in a broader detection range. The study also highlights the potential for further optimization by increasing the structural robustness of the polymer film without compromising flexibility. This versatile and adaptable synthetic design framework holds promise for broader applications in glucose sensing and other analytes.This study presents a non-enzymatic glucose sensing platform using a newly synthesized functionalized monomer, EDOT-PBA, which integrates electrically conducting and receptor moieties within a single organic component. The electrodeposition technique was used to produce two distinct polymer film architectures: pristine PEDOT-PBA (NIP) and molecularly imprinted PEDOT-PBA (MIP). Both architectures demonstrated proficient glucose binding and signal transduction capabilities. Notably, the MIP architecture showed faster stabilization upon glucose uptake, a lower limit of detection, a lower standard deviation, and a broader linear response range compared to the NIP. The MIP architecture's advantages include enhanced film uniformity, improved reproducibility, and a more straightforward analysis in a broader detection range. The study also highlights the potential for further optimization by increasing the structural robustness of the polymer film without compromising flexibility. This versatile and adaptable synthetic design framework holds promise for broader applications in glucose sensing and other analytes.