Single-cell nascent RNA sequencing (scGRO-seq) reveals coordinated global transcription. This study introduces a new method for single-cell nascent RNA sequencing that uses click chemistry to assess genome-wide transcription in individual cells. scGRO-seq enables the simultaneous quantification of transcription in genes and enhancers, and provides insights into the dynamic nature of transcription and the role of enhancers in gene expression. The method uses copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) to label nascent RNA and allows for the identification of networks of enhancers and genes. The results suggest that transcription bursts at super-enhancers precede bursts from associated genes. scGRO-seq can estimate burst size and frequency by directly quantifying transcribing RNA polymerases in individual cells and can leverage replication-dependent non-polyadenylated histone gene transcription to elucidate cell cycle dynamics. The single-nucleotide spatial and temporal resolution of scGRO-seq enables the identification of networks of enhancers and genes. The study also reveals the co-transcription of functionally related genes and the coordination between enhancer-gene pairs. scGRO-seq provides a comprehensive view of the dynamic nature of transcription and the origin and propagation of transcription signals. The method has potential applications in understanding gene regulation and in clinical settings for studying transcriptional dynamics and regulatory mechanisms. The study highlights the importance of enhancers in gene expression and the role of transcriptional bursting in gene regulation. The results demonstrate the ability of scGRO-seq to investigate the mechanisms of transcription regulation and the role of enhancers in gene expression. The study also shows that transcription is a discontinuous process characterized by short bursts and long inter-burst silent periods. The findings have implications for understanding the mechanisms of transcription regulation during the cell cycle, development and disease. The study provides insights into the functional association of enhancers and genes and the dynamic nature of global transcription. The results suggest that the bursting of transcription at super-enhancers precedes bursting from associated genes. The study also reveals the co-transcription of functionally related genes and the coordination between enhancer-gene pairs. The method has potential applications in understanding gene regulation and in clinical settings for studying transcriptional dynamics and regulatory mechanisms. The study highlights the importance of enhancers in gene expression and the role of transcriptional bursting in gene regulation. The results demonstrate the ability of scGRO-seq to investigate the mechanisms of transcription regulation and the role of enhancers in gene expression. The study also shows that transcription is a discontinuous process characterized by short bursts and long inter-burst silent periods. The findings have implications for understanding the mechanisms of transcription regulation during the cell cycle, development and disease. The study provides insights into the functional association of enhancers and genes and the dynamic nature of global transcription. The results suggest that the bursting of transcription at super-enhancers precedes bursting from associated genes. The study also reveals the coSingle-cell nascent RNA sequencing (scGRO-seq) reveals coordinated global transcription. This study introduces a new method for single-cell nascent RNA sequencing that uses click chemistry to assess genome-wide transcription in individual cells. scGRO-seq enables the simultaneous quantification of transcription in genes and enhancers, and provides insights into the dynamic nature of transcription and the role of enhancers in gene expression. The method uses copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) to label nascent RNA and allows for the identification of networks of enhancers and genes. The results suggest that transcription bursts at super-enhancers precede bursts from associated genes. scGRO-seq can estimate burst size and frequency by directly quantifying transcribing RNA polymerases in individual cells and can leverage replication-dependent non-polyadenylated histone gene transcription to elucidate cell cycle dynamics. The single-nucleotide spatial and temporal resolution of scGRO-seq enables the identification of networks of enhancers and genes. The study also reveals the co-transcription of functionally related genes and the coordination between enhancer-gene pairs. scGRO-seq provides a comprehensive view of the dynamic nature of transcription and the origin and propagation of transcription signals. The method has potential applications in understanding gene regulation and in clinical settings for studying transcriptional dynamics and regulatory mechanisms. The study highlights the importance of enhancers in gene expression and the role of transcriptional bursting in gene regulation. The results demonstrate the ability of scGRO-seq to investigate the mechanisms of transcription regulation and the role of enhancers in gene expression. The study also shows that transcription is a discontinuous process characterized by short bursts and long inter-burst silent periods. The findings have implications for understanding the mechanisms of transcription regulation during the cell cycle, development and disease. The study provides insights into the functional association of enhancers and genes and the dynamic nature of global transcription. The results suggest that the bursting of transcription at super-enhancers precedes bursting from associated genes. The study also reveals the co-transcription of functionally related genes and the coordination between enhancer-gene pairs. The method has potential applications in understanding gene regulation and in clinical settings for studying transcriptional dynamics and regulatory mechanisms. The study highlights the importance of enhancers in gene expression and the role of transcriptional bursting in gene regulation. The results demonstrate the ability of scGRO-seq to investigate the mechanisms of transcription regulation and the role of enhancers in gene expression. The study also shows that transcription is a discontinuous process characterized by short bursts and long inter-burst silent periods. The findings have implications for understanding the mechanisms of transcription regulation during the cell cycle, development and disease. The study provides insights into the functional association of enhancers and genes and the dynamic nature of global transcription. The results suggest that the bursting of transcription at super-enhancers precedes bursting from associated genes. The study also reveals the co