Patho-DBiT enables spatial whole transcriptome sequencing in clinical FFPE tissues, allowing for the simultaneous profiling of gene expression, splicing, and non-coding RNAs. It enables genome-wide detection of spatial SNV distribution to distinguish malignant subclones and dissect spatiotemporal dynamics of lymphomagenesis at the single-cell level. Patho-DBiT is a platform that enables spatial profiling of a range of RNA species in clinical FFPE tissues with high sensitivity and coverage. It allows for genome-wide mapping of single-nucleotide RNA variants to distinguish subclones and dissect spatiotemporal tumorigenesis at the cellular level. Patho-DBiT maps microRNA regulatory networks and RNA splicing dynamics, decoding their roles in spatial tumorigenesis. Single-cell level Patho-DBiT dissects the spatiotemporal cellular dynamics driving tumor clonal architecture and progression. Patho-DBiT stands poised as a valuable platform to unravel rich RNA biology in FFPE tissues to aid in clinical pathology evaluation. Patho-DBiT is a platform that enables spatial profiling of a range of RNA species in clinical FFPE tissues with high sensitivity and coverage. It allows for genome-wide mapping of single-nucleotide RNA variants to distinguish subclones and dissect spatiotemporal tumorigenesis at the cellular level. Patho-DBiT maps microRNA regulatory networks and RNA splicing dynamics, decoding their roles in spatial tumorigenesis. Single-cell level Patho-DBiT dissects the spatiotemporal cellular dynamics driving tumor clonal architecture and progression. Patho-DBiT stands poised as a valuable platform to unravel rich RNA biology in FFPE tissues to aid in clinical pathology evaluation. Patho-DBiT enables spatial profiling of a range of RNA species in clinical FFPE tissues with high sensitivity and coverage. It allows for genome-wide mapping of single-nucleotide RNA variants to distinguish subclones and dissect spatiotemporal tumorigenesis at the cellular level. Patho-DBiT maps microRNA regulatory networks and RNA splicing dynamics, decoding their roles in spatial tumorigenesis. Single-cell level Patho-DBiT dissects the spatiotemporal cellular dynamics driving tumor clonal architecture and progression. Patho-DBiT stands poised as a valuable platform to unravel rich RNA biology in FFPE tissues to aid in clinical pathology evaluation.Patho-DBiT enables spatial whole transcriptome sequencing in clinical FFPE tissues, allowing for the simultaneous profiling of gene expression, splicing, and non-coding RNAs. It enables genome-wide detection of spatial SNV distribution to distinguish malignant subclones and dissect spatiotemporal dynamics of lymphomagenesis at the single-cell level. Patho-DBiT is a platform that enables spatial profiling of a range of RNA species in clinical FFPE tissues with high sensitivity and coverage. It allows for genome-wide mapping of single-nucleotide RNA variants to distinguish subclones and dissect spatiotemporal tumorigenesis at the cellular level. Patho-DBiT maps microRNA regulatory networks and RNA splicing dynamics, decoding their roles in spatial tumorigenesis. Single-cell level Patho-DBiT dissects the spatiotemporal cellular dynamics driving tumor clonal architecture and progression. Patho-DBiT stands poised as a valuable platform to unravel rich RNA biology in FFPE tissues to aid in clinical pathology evaluation. Patho-DBiT is a platform that enables spatial profiling of a range of RNA species in clinical FFPE tissues with high sensitivity and coverage. It allows for genome-wide mapping of single-nucleotide RNA variants to distinguish subclones and dissect spatiotemporal tumorigenesis at the cellular level. Patho-DBiT maps microRNA regulatory networks and RNA splicing dynamics, decoding their roles in spatial tumorigenesis. Single-cell level Patho-DBiT dissects the spatiotemporal cellular dynamics driving tumor clonal architecture and progression. Patho-DBiT stands poised as a valuable platform to unravel rich RNA biology in FFPE tissues to aid in clinical pathology evaluation. Patho-DBiT enables spatial profiling of a range of RNA species in clinical FFPE tissues with high sensitivity and coverage. It allows for genome-wide mapping of single-nucleotide RNA variants to distinguish subclones and dissect spatiotemporal tumorigenesis at the cellular level. Patho-DBiT maps microRNA regulatory networks and RNA splicing dynamics, decoding their roles in spatial tumorigenesis. Single-cell level Patho-DBiT dissects the spatiotemporal cellular dynamics driving tumor clonal architecture and progression. Patho-DBiT stands poised as a valuable platform to unravel rich RNA biology in FFPE tissues to aid in clinical pathology evaluation.