This chapter, authored by J. Gutierrez, provides detailed methods for preserving, clearing, and mounting Tetranychidae ( spider mites) for microscopic examination. The preservation method involves storing mites in 70% ethyl alcohol in small, hermetically sealed tubes. For clearing, pure lactic acid colored with lignin pink is recommended to inflate the body and extend the legs, while chitinized cuticles remain intact. Temporary mounts are created using a cavity ground in glass slides, where mites are placed in colored lactic acid and covered with a thin square cover glass. Permanent mounts can be made using Hoyer's solution, which requires heating and careful removal of air bubbles. Both temporary and permanent mounts should be labeled with information about the host plant, collection details, and species characteristics. The chapter also includes references to key studies and techniques in the field.This chapter, authored by J. Gutierrez, provides detailed methods for preserving, clearing, and mounting Tetranychidae ( spider mites) for microscopic examination. The preservation method involves storing mites in 70% ethyl alcohol in small, hermetically sealed tubes. For clearing, pure lactic acid colored with lignin pink is recommended to inflate the body and extend the legs, while chitinized cuticles remain intact. Temporary mounts are created using a cavity ground in glass slides, where mites are placed in colored lactic acid and covered with a thin square cover glass. Permanent mounts can be made using Hoyer's solution, which requires heating and careful removal of air bubbles. Both temporary and permanent mounts should be labeled with information about the host plant, collection details, and species characteristics. The chapter also includes references to key studies and techniques in the field.