This review discusses the role of biomolecular condensates, also known as membraneless organelles (MLOs), in the regulation of alternative splicing and their implications in various human diseases. The authors highlight how alternative splicing influences the formation and properties of condensates, and how these condensates反过来影响splicing regulation. They also explore the spatial connections between splicing regulation and nuclear bodies such as transcriptional condensates, splicing condensates, and nuclear speckles. The review further delves into how splicing regulation through biomolecular condensates is implicated in neurodegenerative diseases, cancer, developmental disorders, and cardiomyopathies. Finally, it addresses open questions about the roles of condensates and MLOs in splicing regulation and suggests experimental approaches to study them.This review discusses the role of biomolecular condensates, also known as membraneless organelles (MLOs), in the regulation of alternative splicing and their implications in various human diseases. The authors highlight how alternative splicing influences the formation and properties of condensates, and how these condensates反过来影响splicing regulation. They also explore the spatial connections between splicing regulation and nuclear bodies such as transcriptional condensates, splicing condensates, and nuclear speckles. The review further delves into how splicing regulation through biomolecular condensates is implicated in neurodegenerative diseases, cancer, developmental disorders, and cardiomyopathies. Finally, it addresses open questions about the roles of condensates and MLOs in splicing regulation and suggests experimental approaches to study them.