This study develops a de novo peptide nanozyme that mimics the dual antifungal actions of antimicrobial peptides (AMPs) and antimicrobial enzymes (AMEs). The nanozyme, composed of a heptapeptide (IHHHCI), is designed to assemble into β-sheet nanotubes with high thermostability and resistance to enzymatic degradation. These nanotubes exhibit phospholipase C-like and peroxidase-like activities, demonstrating their ability to perform cascade antifungal actions, including outer mannan docking, wall disruption, lipid peroxidation, and subsequent ferroptotic death. The nanozyme effectively kills over 90% of *Candida albicans* within 10 minutes on disinfection pads, showcasing its potential as an effective antimicrobial agent. The study highlights the successful integration of AMPs and AMEs' antimicrobial mechanisms into a single nanozyme, offering a promising strategy for developing multi-antimicrobial materials.This study develops a de novo peptide nanozyme that mimics the dual antifungal actions of antimicrobial peptides (AMPs) and antimicrobial enzymes (AMEs). The nanozyme, composed of a heptapeptide (IHHHCI), is designed to assemble into β-sheet nanotubes with high thermostability and resistance to enzymatic degradation. These nanotubes exhibit phospholipase C-like and peroxidase-like activities, demonstrating their ability to perform cascade antifungal actions, including outer mannan docking, wall disruption, lipid peroxidation, and subsequent ferroptotic death. The nanozyme effectively kills over 90% of *Candida albicans* within 10 minutes on disinfection pads, showcasing its potential as an effective antimicrobial agent. The study highlights the successful integration of AMPs and AMEs' antimicrobial mechanisms into a single nanozyme, offering a promising strategy for developing multi-antimicrobial materials.