The article discusses the development of a modified Ziehl-Neelsen staining technique for the detection of Cryptosporidium in fecal smears and intestinal scrapings. Cryptosporidium, known to cause neonatal diarrhea in calves and other animals, is challenging to identify due to its similar appearance to yeasts under Giemsa staining. The modified staining method involves a series of steps, including fixation, staining with carbol fuchsin, differentiation with H₂SO₄, and counterstaining with malachite green. This technique effectively distinguishes Cryptosporidium from yeasts, allowing for easier detection at low magnifications. The method is reliable and simpler than flotation techniques, making it a valuable tool for routine diagnosis.The article discusses the development of a modified Ziehl-Neelsen staining technique for the detection of Cryptosporidium in fecal smears and intestinal scrapings. Cryptosporidium, known to cause neonatal diarrhea in calves and other animals, is challenging to identify due to its similar appearance to yeasts under Giemsa staining. The modified staining method involves a series of steps, including fixation, staining with carbol fuchsin, differentiation with H₂SO₄, and counterstaining with malachite green. This technique effectively distinguishes Cryptosporidium from yeasts, allowing for easier detection at low magnifications. The method is reliable and simpler than flotation techniques, making it a valuable tool for routine diagnosis.