This article by Michael L. Watson, published in 1958, describes the application of heavy metal stains (lead and barium hydroxides) for electron microscopy of tissue sections. The methods for preparing and staining liver sections are detailed, including the use of OsO4 fixation, n-butyl methacrylate embedding, and carbon-coated grids. Lead acetate, barium hydroxide, and lead hydroxide solutions are prepared and their staining properties are discussed. Lead acetate stains membranes, RNA-containing particles, and other cellular components, while barium hydroxide emphasizes structures within the Golgi region and lipid inclusion bodies. Lead hydroxide stains irregularly shaped particles in hepatic cells and RNA-containing particles uniformly. The article also discusses the interpretation of staining results and the potential for differential extraction and stabilization of tissue components during staining. The authors note that lead hydroxide is particularly useful due to its broad applicability and high staining intensity.This article by Michael L. Watson, published in 1958, describes the application of heavy metal stains (lead and barium hydroxides) for electron microscopy of tissue sections. The methods for preparing and staining liver sections are detailed, including the use of OsO4 fixation, n-butyl methacrylate embedding, and carbon-coated grids. Lead acetate, barium hydroxide, and lead hydroxide solutions are prepared and their staining properties are discussed. Lead acetate stains membranes, RNA-containing particles, and other cellular components, while barium hydroxide emphasizes structures within the Golgi region and lipid inclusion bodies. Lead hydroxide stains irregularly shaped particles in hepatic cells and RNA-containing particles uniformly. The article also discusses the interpretation of staining results and the potential for differential extraction and stabilization of tissue components during staining. The authors note that lead hydroxide is particularly useful due to its broad applicability and high staining intensity.