The article discusses the standardization of sample collection, isolation, and analysis methods in extracellular vesicle (EV) research. It highlights the growing interest in EV as biomarkers and therapeutic targets, particularly in oncology. The International Society for Extracellular Vesicles (ISEV) organized a workshop in 2012 to address technical issues and standardize methods for EV isolation and analysis. The article reviews the outcomes of these discussions, emphasizing the need for standardized protocols, appropriate controls, and rigorous study designs. It also provides detailed guidelines for sample collection and processing, including specific considerations for different biological fluids such as blood, saliva, urine, breast milk, and cerebrospinal fluid. The article underscores the importance of minimizing contamination, standardizing techniques, and ensuring comparability across studies. Additionally, it addresses the challenges of sample size determination, data submission, and ethical considerations in EV research.The article discusses the standardization of sample collection, isolation, and analysis methods in extracellular vesicle (EV) research. It highlights the growing interest in EV as biomarkers and therapeutic targets, particularly in oncology. The International Society for Extracellular Vesicles (ISEV) organized a workshop in 2012 to address technical issues and standardize methods for EV isolation and analysis. The article reviews the outcomes of these discussions, emphasizing the need for standardized protocols, appropriate controls, and rigorous study designs. It also provides detailed guidelines for sample collection and processing, including specific considerations for different biological fluids such as blood, saliva, urine, breast milk, and cerebrospinal fluid. The article underscores the importance of minimizing contamination, standardizing techniques, and ensuring comparability across studies. Additionally, it addresses the challenges of sample size determination, data submission, and ethical considerations in EV research.