Staphylococcus aureus (S. aureus) is a significant pathogen in animal husbandry, causing various diseases such as mastitis, skin infections, and arthritis. The formation of biofilms exacerbates S. aureus infections by allowing bacteria to adhere to surfaces and persist in animals, leading to health crises and safety issues in livestock products. This review discusses the harm caused by S. aureus and its biofilms, the transmission pathways, and the molecular mechanisms of biofilm formation. It also explores strategies for removing and eradicating S. aureus and biofilms, including the use of antimicrobial peptides, plant extracts, nanoparticles, phages, and antibodies. These strategies aim to maintain livestock health, improve productivity, and ensure the sustainable development of animal husbandry while ensuring the safety of livestock products. The review highlights the challenges posed by antibiotic resistance and the need for alternative treatments to address biofilm-related infections.Staphylococcus aureus (S. aureus) is a significant pathogen in animal husbandry, causing various diseases such as mastitis, skin infections, and arthritis. The formation of biofilms exacerbates S. aureus infections by allowing bacteria to adhere to surfaces and persist in animals, leading to health crises and safety issues in livestock products. This review discusses the harm caused by S. aureus and its biofilms, the transmission pathways, and the molecular mechanisms of biofilm formation. It also explores strategies for removing and eradicating S. aureus and biofilms, including the use of antimicrobial peptides, plant extracts, nanoparticles, phages, and antibodies. These strategies aim to maintain livestock health, improve productivity, and ensure the sustainable development of animal husbandry while ensuring the safety of livestock products. The review highlights the challenges posed by antibiotic resistance and the need for alternative treatments to address biofilm-related infections.