The paper by G. N. Wilkinson discusses the application of statistical methods in enzyme kinetics, particularly focusing on regression analysis. While graphical methods like the double-reciprocal plot are commonly used to estimate kinetic parameters, they do not provide measures of precision. The paper introduces weighted and non-linear regression methods to address this issue, providing detailed computational methods for estimating the Michaelis-Menten parameters \(K_m\) and \(V\), along with their standard errors. These methods are illustrated through examples, including the estimation of dissociation constants from a series of \(K_m\) determinations. The author emphasizes the importance of statistical methods in avoiding subjective biases and providing more accurate and precise estimates. The paper also discusses the advantages of using linear plots over double-reciprocal plots for graphical determinations of \(K_m\) and \(V\). Overall, the statistical approaches enhance the reliability and accuracy of enzyme kinetic studies.The paper by G. N. Wilkinson discusses the application of statistical methods in enzyme kinetics, particularly focusing on regression analysis. While graphical methods like the double-reciprocal plot are commonly used to estimate kinetic parameters, they do not provide measures of precision. The paper introduces weighted and non-linear regression methods to address this issue, providing detailed computational methods for estimating the Michaelis-Menten parameters \(K_m\) and \(V\), along with their standard errors. These methods are illustrated through examples, including the estimation of dissociation constants from a series of \(K_m\) determinations. The author emphasizes the importance of statistical methods in avoiding subjective biases and providing more accurate and precise estimates. The paper also discusses the advantages of using linear plots over double-reciprocal plots for graphical determinations of \(K_m\) and \(V\). Overall, the statistical approaches enhance the reliability and accuracy of enzyme kinetic studies.