Nature Portfolio aims to enhance the reproducibility of published research through structured and transparent reporting. The document outlines specific guidelines for statistical analyses, software and code availability, data availability, and research involving human participants. For statistical analyses, authors must provide details such as sample size, statistical tests used, and assumptions or corrections. Software and code must be made available, especially for custom algorithms, and data availability statements must be included. Manuscripts involving human participants must adhere to specific policies and provide full approval details. Additionally, the document provides field-specific reporting guidelines for life sciences, behavioral and social sciences, and ecological, evolutionary, and environmental sciences. Authors are required to disclose relevant materials, systems, and methods used in their studies.Nature Portfolio aims to enhance the reproducibility of published research through structured and transparent reporting. The document outlines specific guidelines for statistical analyses, software and code availability, data availability, and research involving human participants. For statistical analyses, authors must provide details such as sample size, statistical tests used, and assumptions or corrections. Software and code must be made available, especially for custom algorithms, and data availability statements must be included. Manuscripts involving human participants must adhere to specific policies and provide full approval details. Additionally, the document provides field-specific reporting guidelines for life sciences, behavioral and social sciences, and ecological, evolutionary, and environmental sciences. Authors are required to disclose relevant materials, systems, and methods used in their studies.