The study investigates the potential of a strontium-containing piezoelectric biofilm to promote dentin tissue regeneration. The biofilm, composed of P(VDF-TrFE) with 2 wt% SrCl₂, exhibits high flexibility, biocompatibility, and a large piezoelectric coefficient (d₃₃ = 14 pC N⁻¹). This property creates an electric microenvironment that recruits dental pulp stem cells (DPSCs) and induces their differentiation into odontoblasts, which are essential for dentin regeneration. Strontium ions released from the film further enhance this process. In vitro experiments show that the biofilm recruits DPSCs, induces odonto-differentiation, and promotes mineralized matrix secretion. In vivo studies in dog models demonstrate that the biofilm effectively induces dentin tissue regeneration, forming new dentin and sealing the pulp. The piezoelectric biofilm shows promise for improving dentin tissue repair in clinical settings, offering a therapeutic approach to treat dentin defects and related dental diseases.The study investigates the potential of a strontium-containing piezoelectric biofilm to promote dentin tissue regeneration. The biofilm, composed of P(VDF-TrFE) with 2 wt% SrCl₂, exhibits high flexibility, biocompatibility, and a large piezoelectric coefficient (d₃₃ = 14 pC N⁻¹). This property creates an electric microenvironment that recruits dental pulp stem cells (DPSCs) and induces their differentiation into odontoblasts, which are essential for dentin regeneration. Strontium ions released from the film further enhance this process. In vitro experiments show that the biofilm recruits DPSCs, induces odonto-differentiation, and promotes mineralized matrix secretion. In vivo studies in dog models demonstrate that the biofilm effectively induces dentin tissue regeneration, forming new dentin and sealing the pulp. The piezoelectric biofilm shows promise for improving dentin tissue repair in clinical settings, offering a therapeutic approach to treat dentin defects and related dental diseases.