Sphingolipid- and cholesterol-rich membrane rafts are lipid domains in cell membranes that have distinct physical properties, such as tight acyl chain packing and a higher melting temperature (Tm) compared to other membrane lipids. These rafts are thought to exist in a liquid-ordered (Lo) phase and are enriched in sphingolipids and cholesterol. Rafts are important for cellular functions, particularly in signaling in hematopoietic cells. They are often found in detergent-insoluble membranes (DRMs), which can be isolated from cell lysates and are believed to be derived from rafts. The presence of rafts is supported by their detergent insolubility and their association with specific proteins and lipids. Rafts may also play a role in the clustering and localization of proteins, which is essential for signaling processes. However, the exact structure and function of rafts remain areas of active research, with challenges in visualizing them due to their small size and low concentration. Rafts are likely to be most abundant in membranes rich in cholesterol and sphingolipids, such as the plasma membrane, late secretory pathway, and endocytic compartments. Rafts may also be involved in various cellular processes, including signal transduction, pathogen interaction, and cell adhesion. The study of rafts has provided insights into the organization and function of cell membranes, particularly in hematopoietic cells.Sphingolipid- and cholesterol-rich membrane rafts are lipid domains in cell membranes that have distinct physical properties, such as tight acyl chain packing and a higher melting temperature (Tm) compared to other membrane lipids. These rafts are thought to exist in a liquid-ordered (Lo) phase and are enriched in sphingolipids and cholesterol. Rafts are important for cellular functions, particularly in signaling in hematopoietic cells. They are often found in detergent-insoluble membranes (DRMs), which can be isolated from cell lysates and are believed to be derived from rafts. The presence of rafts is supported by their detergent insolubility and their association with specific proteins and lipids. Rafts may also play a role in the clustering and localization of proteins, which is essential for signaling processes. However, the exact structure and function of rafts remain areas of active research, with challenges in visualizing them due to their small size and low concentration. Rafts are likely to be most abundant in membranes rich in cholesterol and sphingolipids, such as the plasma membrane, late secretory pathway, and endocytic compartments. Rafts may also be involved in various cellular processes, including signal transduction, pathogen interaction, and cell adhesion. The study of rafts has provided insights into the organization and function of cell membranes, particularly in hematopoietic cells.