Stochastic Optical Reconstruction Microscopy (STORM) is a high-resolution fluorescence microscopy technique that achieves sub-diffraction-limit image resolution by localizing photo-switchable fluorophores with nanometer accuracy. In each imaging cycle, only a fraction of the fluorophores are turned on, allowing their positions to be determined accurately. By repeating this process across multiple cycles, the positions of many fluorophores can be determined, and an overall image is reconstructed. The technique demonstrates an imaging resolution of approximately 20 nm, significantly improving upon the diffraction limit of conventional fluorescence microscopy. STORM uses a cyanine dye pair, Cy5 and Cy3, which can be switched between fluorescent and dark states using different wavelengths of light. The localization accuracy of individual switches is limited by the number of photons emitted per switching cycle, with a theoretical localization accuracy of 4 nm predicted for the cyanine switch. STORM has been successfully applied to resolve structures such as DNA and RecA filaments, showing its potential for high-resolution biological imaging.Stochastic Optical Reconstruction Microscopy (STORM) is a high-resolution fluorescence microscopy technique that achieves sub-diffraction-limit image resolution by localizing photo-switchable fluorophores with nanometer accuracy. In each imaging cycle, only a fraction of the fluorophores are turned on, allowing their positions to be determined accurately. By repeating this process across multiple cycles, the positions of many fluorophores can be determined, and an overall image is reconstructed. The technique demonstrates an imaging resolution of approximately 20 nm, significantly improving upon the diffraction limit of conventional fluorescence microscopy. STORM uses a cyanine dye pair, Cy5 and Cy3, which can be switched between fluorescent and dark states using different wavelengths of light. The localization accuracy of individual switches is limited by the number of photons emitted per switching cycle, with a theoretical localization accuracy of 4 nm predicted for the cyanine switch. STORM has been successfully applied to resolve structures such as DNA and RecA filaments, showing its potential for high-resolution biological imaging.