This review explores the synergistic antibacterial mechanism of silver-copper bimetallic nanoparticles (Ag-Cu NPs) and their potential in combating antibiotic resistance. The excessive use of antibiotics has led to the rapid development of bacterial resistance, posing a significant challenge to public health. Ag-Cu NPs, composed of silver and copper nanoparticles, exhibit strong antibacterial effects with low cytotoxicity, making them promising alternatives to traditional antibiotics. The antibacterial mechanisms of Ag NPs and Cu NPs include the release of metal ions, oxidative stress, and damage to cell integrity. However, the potential cytotoxicity and genotoxicity of these nanoparticles must be considered. Bimetallic nanoparticles offer a solution by mitigating genotoxicity while enhancing antibacterial efficacy. The review discusses various synthesis methods for Ag-Cu NPs, emphasizing their synergistic effects, reactive oxygen species (ROS) generation, photocatalytic properties, and antibacterial mechanisms. The paper also highlights the factors influencing their performance, such as synthesis methods, structures, carriers, pH, and photocatalytic mechanisms. The review concludes that Ag-Cu NPs have the potential to enhance efficacy, reduce toxicity, and find broader applications in combating antibiotic resistance while promoting public health. The study also examines the impact of different synthesis methods, structures, and environmental factors on the antibacterial performance of Ag-Cu NPs. The review emphasizes the importance of understanding the antibacterial mechanisms and factors influencing the performance of Ag-Cu NPs to develop more effective antimicrobial materials.This review explores the synergistic antibacterial mechanism of silver-copper bimetallic nanoparticles (Ag-Cu NPs) and their potential in combating antibiotic resistance. The excessive use of antibiotics has led to the rapid development of bacterial resistance, posing a significant challenge to public health. Ag-Cu NPs, composed of silver and copper nanoparticles, exhibit strong antibacterial effects with low cytotoxicity, making them promising alternatives to traditional antibiotics. The antibacterial mechanisms of Ag NPs and Cu NPs include the release of metal ions, oxidative stress, and damage to cell integrity. However, the potential cytotoxicity and genotoxicity of these nanoparticles must be considered. Bimetallic nanoparticles offer a solution by mitigating genotoxicity while enhancing antibacterial efficacy. The review discusses various synthesis methods for Ag-Cu NPs, emphasizing their synergistic effects, reactive oxygen species (ROS) generation, photocatalytic properties, and antibacterial mechanisms. The paper also highlights the factors influencing their performance, such as synthesis methods, structures, carriers, pH, and photocatalytic mechanisms. The review concludes that Ag-Cu NPs have the potential to enhance efficacy, reduce toxicity, and find broader applications in combating antibiotic resistance while promoting public health. The study also examines the impact of different synthesis methods, structures, and environmental factors on the antibacterial performance of Ag-Cu NPs. The review emphasizes the importance of understanding the antibacterial mechanisms and factors influencing the performance of Ag-Cu NPs to develop more effective antimicrobial materials.