The excessive use of antibiotics has led to the rapid evolution of bacterial resistance, posing a significant challenge to the medical community. To address this issue, the development of new antibacterial materials, such as metallic and metal oxide nanoparticles (MeO NPs), has gained attention. Silver nanoparticles (Ag NPs) and copper nanoparticles (Cu NPs) have been extensively studied for their strong antibacterial properties, including the release of metal ions, oxidative stress responses, and immunostimulatory effects. However, the cytotoxicity and genotoxicity of these nanoparticles are also crucial concerns. Bimetallic nanoparticles (BNPs) composed of Ag NPs and Cu NPs have shown strong antibacterial effects while maintaining low cytotoxicity. These nanoparticles offer an effective means to mitigate genotoxicity and enhance antibacterial efficacy. This paper reviews various synthesis methods for Ag-Cu NPs, emphasizing their synergistic effects, processes of reactive oxygen species (ROS) generation, photocatalytic properties, and antibacterial mechanisms. The synthesis methods discussed include chemical dealloying, pulsed laser ablation, chemical reduction, liquid phase pulsed plasma, microwave, sol-gel, and biosynthetic approaches. The paper also explores the different structures of Ag-Cu NPs, such as core-shell and hybrid structures, and their impact on antibacterial performance. The antibacterial mechanisms of Ag-Cu NPs are influenced by factors such as pH, optical and electrical properties, and the presence of carriers. The synergistic effect of Ag-Cu NPs enhances their antibacterial efficacy by increasing the surface-to-volume ratio, accelerating ion release, and intensifying ROS content and oxidative stress reactions. The low toxicity and genotoxicity of Ag-Cu NPs make them promising candidates for combating antibiotic resistance and promoting public health.The excessive use of antibiotics has led to the rapid evolution of bacterial resistance, posing a significant challenge to the medical community. To address this issue, the development of new antibacterial materials, such as metallic and metal oxide nanoparticles (MeO NPs), has gained attention. Silver nanoparticles (Ag NPs) and copper nanoparticles (Cu NPs) have been extensively studied for their strong antibacterial properties, including the release of metal ions, oxidative stress responses, and immunostimulatory effects. However, the cytotoxicity and genotoxicity of these nanoparticles are also crucial concerns. Bimetallic nanoparticles (BNPs) composed of Ag NPs and Cu NPs have shown strong antibacterial effects while maintaining low cytotoxicity. These nanoparticles offer an effective means to mitigate genotoxicity and enhance antibacterial efficacy. This paper reviews various synthesis methods for Ag-Cu NPs, emphasizing their synergistic effects, processes of reactive oxygen species (ROS) generation, photocatalytic properties, and antibacterial mechanisms. The synthesis methods discussed include chemical dealloying, pulsed laser ablation, chemical reduction, liquid phase pulsed plasma, microwave, sol-gel, and biosynthetic approaches. The paper also explores the different structures of Ag-Cu NPs, such as core-shell and hybrid structures, and their impact on antibacterial performance. The antibacterial mechanisms of Ag-Cu NPs are influenced by factors such as pH, optical and electrical properties, and the presence of carriers. The synergistic effect of Ag-Cu NPs enhances their antibacterial efficacy by increasing the surface-to-volume ratio, accelerating ion release, and intensifying ROS content and oxidative stress reactions. The low toxicity and genotoxicity of Ag-Cu NPs make them promising candidates for combating antibiotic resistance and promoting public health.