Gold nanoparticles (AuNPs) are promising antioxidants due to their minimal toxicity, simple synthesis, and detectability. They exhibit antioxidant properties by scavenging free radicals, enhancing their potential in healthcare applications such as anti-aging, anti-inflammatory, and wound healing. This review highlights recent advancements in the synthesis of AuNPs as antioxidants, methods for assessing their antioxidant capacity, and their mechanisms of action. AuNPs are categorized into top-down and bottom-up approaches, with the latter being more favorable for antioxidant applications due to its advantages like minimal waste and high stability. Various synthesis methods, including chemical, biological, and physical techniques, have been explored to produce AuNPs with antioxidant properties. Biological methods, such as using plant extracts, bacteria, fungi, and algae, are effective in synthesizing AuNPs with high antioxidant activity. The antioxidant capacity of AuNPs is evaluated using methods like DPPH, ABTS, and hydroxyl radical scavenging. In vivo studies in animal models, including rats, mice, and rabbits, demonstrate the antioxidant effects of AuNPs in managing conditions like diabetes, oxidative stress, and inflammation. The antioxidant activity of AuNPs is influenced by their physicochemical properties, such as size, shape, and surface chemistry. AuNPs have potential applications in skin aging, wound healing, and treating pathological conditions like atherosclerosis and inflammatory diseases. Their unique properties make them suitable for clinical applications as effective antioxidants.Gold nanoparticles (AuNPs) are promising antioxidants due to their minimal toxicity, simple synthesis, and detectability. They exhibit antioxidant properties by scavenging free radicals, enhancing their potential in healthcare applications such as anti-aging, anti-inflammatory, and wound healing. This review highlights recent advancements in the synthesis of AuNPs as antioxidants, methods for assessing their antioxidant capacity, and their mechanisms of action. AuNPs are categorized into top-down and bottom-up approaches, with the latter being more favorable for antioxidant applications due to its advantages like minimal waste and high stability. Various synthesis methods, including chemical, biological, and physical techniques, have been explored to produce AuNPs with antioxidant properties. Biological methods, such as using plant extracts, bacteria, fungi, and algae, are effective in synthesizing AuNPs with high antioxidant activity. The antioxidant capacity of AuNPs is evaluated using methods like DPPH, ABTS, and hydroxyl radical scavenging. In vivo studies in animal models, including rats, mice, and rabbits, demonstrate the antioxidant effects of AuNPs in managing conditions like diabetes, oxidative stress, and inflammation. The antioxidant activity of AuNPs is influenced by their physicochemical properties, such as size, shape, and surface chemistry. AuNPs have potential applications in skin aging, wound healing, and treating pathological conditions like atherosclerosis and inflammatory diseases. Their unique properties make them suitable for clinical applications as effective antioxidants.