TIMER2.0 is an enhanced web server for analyzing tumor-infiltrating immune cells, designed to improve the estimation of immune cell infiltration levels and facilitate the investigation of complex tumor-immune interactions. The platform integrates six state-of-the-art algorithms (TIMER, xCell, MCP-counter, CIBERSORT, EPIC, and quanTIseq) to provide more robust and comprehensive results. TIMER2.0 offers four modules for exploring associations between immune infiltrates and genetic or clinical features, and four modules for investigating cancer-related associations in The Cancer Genome Atlas (TCGA) cohorts. Each module generates a functional heatmap table, enabling users to identify significant associations across multiple cancer types. The platform also includes an estimation component for inferring immune cell infiltration in user-provided expression profiles. TIMER2.0 is freely available and aims to support cancer immunology research by providing comprehensive analysis and visualization tools for tumor-infiltrating immune cells.TIMER2.0 is an enhanced web server for analyzing tumor-infiltrating immune cells, designed to improve the estimation of immune cell infiltration levels and facilitate the investigation of complex tumor-immune interactions. The platform integrates six state-of-the-art algorithms (TIMER, xCell, MCP-counter, CIBERSORT, EPIC, and quanTIseq) to provide more robust and comprehensive results. TIMER2.0 offers four modules for exploring associations between immune infiltrates and genetic or clinical features, and four modules for investigating cancer-related associations in The Cancer Genome Atlas (TCGA) cohorts. Each module generates a functional heatmap table, enabling users to identify significant associations across multiple cancer types. The platform also includes an estimation component for inferring immune cell infiltration in user-provided expression profiles. TIMER2.0 is freely available and aims to support cancer immunology research by providing comprehensive analysis and visualization tools for tumor-infiltrating immune cells.